Onchocerca ochengi male worms implanted in SCID mice and Gerbil: Relationship between microfilaridermia status of cows, nodular worm viability and fertility and worm survival in the rodents.

Autor: Akumtoh DN; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon; Parasite and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon. Electronic address: nkimdesmond@yahoo.fr., Njouendou AJ; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon; Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, Buea, Cameroon. Electronic address: ajnjouendou@gmail.com., Metuge HM; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon; Parasite and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon. Electronic address: hanna.sjoberg@nds.ox.ac.uk., Sjoberg HT; Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, UK., Pionnier NP; Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, UK. Electronic address: Nicolas.Pionnier@crl.com., Chunda VC; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon; Parasite and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon. Electronic address: chundavalerinne@yahoo.com., Gandjui NVT; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon; Parasite and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon. Electronic address: gvictornarcisse@gmail.com., Ndzeshang LB; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon; Parasite and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon. Electronic address: bndzeshang@yahoo.com., Fombad FF; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon; Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, Buea, Cameroon. Electronic address: ffffombad@gmail.com., Abong RA; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon; Parasite and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon. Electronic address: abongr@gmail.com., Enyong PA; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon; Parasite and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon. Electronic address: enyongap@gmail.com., Fru-Cho J; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon; Parasite and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon. Electronic address: jerome.frucho.1@gmail.com., Esum ME; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon; Parasite and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon. Electronic address: mathias_mesum@yahoo.fr., Ritter M; Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Germany; German Center for Infection Research (DZIF), Bonn - Cologne Partner Site, Bonn, Germany. Electronic address: Manuel.Ritter@ukbonn.de., Taylor MJ; Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, UK. Electronic address: Mark.Taylor@lstmed.ac.uk., Turner JD; Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, UK. Electronic address: Joseph.Turner@lstmed.ac.uk., Wanji S; Research Foundation for Tropical Diseases and the Environment (REFOTDE), Buea, Cameroon; Parasite and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon. Electronic address: swanji@yahoo.fr.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2021 Oct; Vol. 229, pp. 108143. Date of Electronic Publication: 2021 Aug 24.
DOI: 10.1016/j.exppara.2021.108143
Abstrakt: Background: Current treatment options for onchocerciasis are sub-optimal, prompting research and development of a safe cure (macrofilaricide). Onchocerca ochengi, a parasite of cattle, is used as a close surrogate for the human parasite O. volvulus in a murine model for pre-clinical screening of macrofilaricides. Skin from naturally infected cattle have been used in previous studies as a reliable source of parasite material. However, there is limited knowledge on how source-related factors such as the microfilaridermia status of the cattle, the nodule load and nodular worm viability may affect survival of male O. ochengi worms implanted in the rodent hosts. Such relationships were investigated in this study.
Methods: Dermal tissue and nodules were obtained from Gudali cattle, dissected and cultured to obtain migrating microfilariae (mf) and male worms. Emerged male worms were implanted into SCID mice and Gerbils (Meriones unguiculatus) and recovery rates were determined upon 42 days post implantation. Finally, nodules were processed for histology and embryogram analyses to assess the nodular worm viability and fertility, respectively.
Results: Of the 69 cattle sampled, 24 (34.8%) were mf + and 45 (65.2%) were mf - . The mean nodule loads were 180.5 ± 117.7 (mf+) and 110.6 ± 102.7 (mf-) (p = 0.0186). The mean male worm harvest from nodules were 76.8 ± 120.3 and 47.2 ± 33.4 (p = 0.2488) for mf + and mf - cattle, respectively. The number of male worms per 100 nodules were 57/100 and 46/100 nodules for mf + and mf - cows, respectively. Female worms from nodules of mf - cows had higher counts of both normal and abnormal embryos with higher proportions of dead nodular worms evinced by histology compared to those from mf + cows. A total of 651 worms were implanted into mice and gerbils, out of which 129 (19.81%) were recovered. Logistic regression analysis indicated that the microfilaridermia status of the cattle (presence of mf) (OR = 4.3319; P = 0.001) is the single most important predictor of the success of male worm recovery after implantation into rodents.
Conclusion: Microfilaridermic cattle provide a promising source of adult O. ochengi. Male worms from this group of cattle have a better success rate of survival in a murine implant model. Nevertheless, in the programmatic point of view, amicrofilaridermic Gudali cattle would still constitute an important source of O. ochengi male worms with relatively good viability after implantation into rodents.
(Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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