Species Identity, Life History, and Geographic Distance Influence Gut Bacterial Communities in Lab-Reared and European Field-Collected Culicoides Biting midges.

Autor: Möhlmann TWR; Laboratory of Entomology, Wageningen University & Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands., Ter Braak CJF; Biometris, Wageningen University & Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands., Te Beest DE; Biometris, Wageningen University & Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands., Hendriks M; Biointeractions and Plant Health, Wageningen University & Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands., Nijhuis EH; Biointeractions and Plant Health, Wageningen University & Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands., Warris S; Bioscience, Wageningen University & Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands., Drolet BS; Agricultural Research Service, Arthropod-Borne Animal Diseases Research Unit, USDA, 1515 College Ave, Manhattan, KS, USA., van Overbeek L; Biointeractions and Plant Health, Wageningen University & Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands., Koenraadt CJM; Laboratory of Entomology, Wageningen University & Research, P.O. Box 16, 6700 AA, Wageningen, The Netherlands. sander.koenraadt@wur.nl.
Jazyk: angličtina
Zdroj: Microbial ecology [Microb Ecol] 2022 Jul; Vol. 84 (1), pp. 267-284. Date of Electronic Publication: 2021 Aug 26.
DOI: 10.1007/s00248-021-01822-8
Abstrakt: Bacteria are part of the insect gut system and influence many physiological traits of their host. Gut bacteria may even reduce or block the transmission of arboviruses in several species of arthropod vectors. Culicoides biting midges are important arboviral vectors of several livestock and wildlife diseases, yet limited information is available on their gut bacterial communities. Addressing this gap will help inform how these communities can be manipulated and ultimately used as novel tools to control pathogens. To assess how bacterial communities change during the life stages of lab-reared C. nubeculosus and C. sonorensis, endosymbiotic bacteria were identified using Illumina sequencing of 16S rRNA and taxonomically characterised. Analyses were conducted to determine how gut bacterial communities in adults are influenced by species identity and geographic distance among biting midge populations. Communities of the two lab-reared Culicoides species significantly changed after pupation and with maturation into 6-day-old adults. Pseudomonas, Burkholderiaceae and Leucobacter bacteria were part of a core community that was trans-stadially transmitted and found throughout their life cycle. Among field-collected biting midges, the bacterial communities were unique for almost each species. Cardinium, Rickettsia and Wolbachia were some of the most abundant bacteria in midges collected from wetlands. Only Pseudomonas was present in high relative abundance in all field-collected species. In this study, species identity, as well as geographic distance, influenced the gut bacterial communities and may partly explain known inter- and intra-species variability in vector competence. Additionally, stably associated bacterial species could be candidates for paratransgenic strategies to control vector-borne pathogens.
(© 2021. The Author(s).)
Databáze: MEDLINE