Persistent human bocavirus 1 infection and tonsillar immune responses.

Autor: Ivaska LE; Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital and University of Turku, Turku, Finland., Silvoniemi A; Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital and University of Turku, Turku, Finland., Palomares O; Swiss Institute of Allergy and Asthma Research, University of Zürich, Davos, Switzerland.; Christine Kühne-Center for Allergy Research and Education, Davos, Switzerland.; Department of Biochemistry and Molecular Biology, School of Chemistry, Complutense University of Madrid, Madrid, Spain., Turunen R; Department of Pediatrics and Adolescent Medicine, Turku University Hospital and University of Turku, Turku, Finland.; Children's Hospital, Helsinki University Hospital and University of Helsinki, Helsinki, Finland., Waris M; Clinical Microbiology, Turku University Hospital, Turku, Finland.; Institute of Biomedicine, University of Turku, Turku, Finland., Mikola E; Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital and University of Turku, Turku, Finland.; Department of Otorhinolaryngology, Satakunta Central Hospital, Pori, Finland., Puhakka T; Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Hospital and University of Turku, Turku, Finland.; Department of Otorhinolaryngology, Satakunta Central Hospital, Pori, Finland., Söderlund-Venermo M; Department of Virology, University of Helsinki, Helsinki, Finland., Akdis M; Swiss Institute of Allergy and Asthma Research, University of Zürich, Davos, Switzerland.; Christine Kühne-Center for Allergy Research and Education, Davos, Switzerland., Akdis CA; Swiss Institute of Allergy and Asthma Research, University of Zürich, Davos, Switzerland.; Christine Kühne-Center for Allergy Research and Education, Davos, Switzerland., Jartti T; Department of Pediatrics and Adolescent Medicine, Turku University Hospital and University of Turku, Turku, Finland.; Department of Pediatrics, Oulu University Hospital and University of Oulu, Oulu, Finland.
Jazyk: angličtina
Zdroj: Clinical and translational allergy [Clin Transl Allergy] 2021 Jul; Vol. 11 (6), pp. e12030.
DOI: 10.1002/clt2.12030
Abstrakt: Background: Persistent human bocavirus 1 (HBoV1) infection is a common finding in patients suffering from chronic tonsillar disease. However, the associations between HBoV1 infection and specific immune reactions are not completely known. We aimed to compare in vivo expression of T-cell cytokines, transcription factors, and type I/III interferons in human tonsils between HBoV1-positive and -negative tonsillectomy patients.
Methods: Tonsil tissue samples, nasopharyngeal aspirate (NPA), and serum samples were obtained from 143 immunocompetent adult and child tonsillectomy patients. HBoV1 and 14 other respiratory viruses were detected in NPAs and tonsil tissues by polymerase chain reaction (PCR). Serology and semi-quantitative PCR were used for diagnosing HBoV1 infections. Expression of 14 cytokines and transcription factors (IFN-α, IFN-β, IFN-γ, IL-10, IL-13, IL-17, IL-28, IL-29, IL-37, TGF-β, FOXP3, GATA3, RORC2, Tbet) was analyzed by quantitative reverse-transcription (RT)-PCR in tonsil tissues.
Results: HBoV1 was detected by PCR in NPA and tonsils from 25 (17%) study patients. Serology results indicated prior nonacute infections in 81% of cases. Tonsillar cytokine responses were affected by HBoV1 infection. The suppression of two transcription factors, RORC2 and FOXP3, was associated with HBoV1 infection (p < 0.05). Furthermore, intratonsillar HBoV1-DNA loads correlated negatively with IFN-λ family cytokines and IL-13.
Conclusions: Our study shows distinctively decreased T-helper 17 and T-regulatory type immune responses in local lymphoid tissue in HBoV1-positive tonsillectomy patients. HBoV1 may act as a suppressive immune modulator.
(© 2021 The Authors. Clinical and Translational Allergy published by John Wiley and Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology.)
Databáze: MEDLINE
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