Iodine-based contrast staining improves micro-computed tomography of atherosclerotic coronary arteries.

Autor:	Self TS; Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX, USA., Ginn-Hedman AM; Biomedical Engineering, Texas A&M University, College Station, TX, USA., Newell-Fugate AE; Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX, USA., Weeks BR; Veterinary Pathobiology, Texas A&M University, College Station, TX, USA., Heaps CL; Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX, USA.; Michael E. DeBakey Institute for Comparative Cardiovascular Science and Biomedical Devices, Texas A&M University, College Station, TX, USA.
Jazyk:	angličtina
Zdroj:	MethodsX [MethodsX] 2021 Mar 05; Vol. 8, pp. 101297. Date of Electronic Publication: 2021 Mar 05 (Print Publication: 2021).
DOI:	10.1016/j.mex.2021.101297
Abstrakt:	We sought to develop a reversible staining protocol using micro-computed tomography (micro-CT) paired with a radiopaque contrast agent that allows for three-dimensional in situ visualization and characterization of atherosclerotic plaques. Atherosclerotic porcine coronary arteries were dissected from surrounding myocardium and incubated in iohexol at various concentrations and incubation times and then imaged using direct radiography. Line profiles were generated across the artery x-ray to determine effectiveness of the radiopaque contrast agent to penetrate the tissue. Our studies revealed that, to sufficiently delineate tissue constructs, the minimum effective iohexol concentration and incubation time were 240 mgI/mL for 1 hour. Among all groups, 24 hours of de-staining brought radiopacity back to control levels. After iohexol incubation, micro-CT was performed. Our findings demonstrate that extended staining times and a minimum iohexol concentration of 240 mgI/mL are required for effective tissue perfusion, which eliminates the diffusion distribution profile inherent to the ability of the contrast agent to traverse tissue layers.•Iohexol enhances ex vivo micro-CT imaging of atherosclerotic coronary arteries•Iohexol allows for improved tissue segmentation during micro-CT image analysis•Effectiveness of iohexol penetration of the tissue was dependent on concentration and duration of incubation. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Author(s).)
Databáze:	MEDLINE
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