Determination of arsenic chemical species in rice grains using high-performance liquid chromatography coupled to hydride generator with atomic fluorescence detector (HPLC-HG-AFS).

Autor: Urango-Cárdenas ID; Universidad de Córdoba, Monteria Carrera 6 Nº 76-103, Córdoba, Monteria Colombia., Burgos-Núñez S; Universidad de Córdoba, Monteria Carrera 6 Nº 76-103, Córdoba, Monteria Colombia., Ospina Herrera LÁ; Universidad de Córdoba, Monteria Carrera 6 Nº 76-103, Córdoba, Monteria Colombia., Enamorado-Montes G; Universidad de Córdoba, Monteria Carrera 6 Nº 76-103, Córdoba, Monteria Colombia., Marrugo-Negrete JL; Universidad de Córdoba, Monteria Carrera 6 Nº 76-103, Córdoba, Monteria Colombia.
Jazyk: angličtina
Zdroj: MethodsX [MethodsX] 2021 Feb 19; Vol. 8, pp. 101281. Date of Electronic Publication: 2021 Feb 19 (Print Publication: 2021).
DOI: 10.1016/j.mex.2021.101281
Abstrakt: The objective of this work is the validation and implementation of an analytical method for the determination of arsenic chemical species in rice grain samples using High-performance liquid chromatography coupled to a hydride generator with atomic fluorescence detector (HPLC-HG-AFS). The extraction protocol developed was based on HNO 3 0.28 M (90 °C, 2 h), microwave-assisted. The results showed recovery percentages of arsenite (As (III)) (99-101%), arsenate (As (V)) (91-96%), dimethylarsinic acid (DMA) (92-102%) and monomethylarsonic acid (MMA) (94-97%). The precision of the method presented coefficients of variation lower than 7% and 8% for repeatability and reproducibility respectively. The detection limits were 2.5, 3.75, 7.5 and 4.0 µg kg -1 for As (III), As (V), DMA and MMA respectively. The proposed methodology is reliable for the quantification of As species, because they are conserved during the extraction.•The extraction protocol developed was based on Microwave-assisted acid extraction.•This methodology offers good sensitivity, precision, accuracy, detection and quantification limits.•It was successfully applied to determination of arsenic chemical species in rice grains.
(© 2021 Universidad de Córdoba, Monteria, Colombia.)
Databáze: MEDLINE
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