Image analysis-based quantification of fungal sporulation by automatic conidia counting and gray value correlation.

Autor:	Muskat LC; University of Applied Sciences Bielefeld, Fermentation and Formulation of Biologicals and Chemicals, Faculty of Engineering Sciences and Mathematics, Bielefeld, Germany.; Georg-August-University Goettingen, Agricultural Entomology, Department of Crop Sciences, Goettingen, Germany., Kerkhoff Y; University of Bielefeld, Faculty of Technology, Biodata Mining Group, Germany.; Free University of Berlin, Institute of Chemistry and Biochemistry, Berlin, Germany., Humbert P; University of Applied Sciences Bielefeld, Fermentation and Formulation of Biologicals and Chemicals, Faculty of Engineering Sciences and Mathematics, Bielefeld, Germany., Nattkemper TW; University of Bielefeld, Faculty of Technology, Biodata Mining Group, Germany., Eilenberg J; University of Copenhagen, Department of Plant and Environmental Sciences, Insect Pathology and Biological Control, Copenhagen, Denmark., Patel AV; University of Applied Sciences Bielefeld, Fermentation and Formulation of Biologicals and Chemicals, Faculty of Engineering Sciences and Mathematics, Bielefeld, Germany.
Jazyk:	angličtina
Zdroj:	MethodsX [MethodsX] 2021 Jan 07; Vol. 8, pp. 101218. Date of Electronic Publication: 2021 Jan 07 (Print Publication: 2021).
DOI:	10.1016/j.mex.2021.101218
Abstrakt:	The present work describes a new computer-assisted image analysis method for the rapid, simple, objective and reproducible quantification of actively discharged fungal spores which can serve as a manual for laboratories working in this context. The method can be used with conventional laboratory equipment by using bright field microscopes, standard scanners and the open-source software ImageJ. Compared to other conidia quantification methods by computer-assisted image analysis, the presented method bears a higher potential to be applied for large-scale sample quantities. The key to make quantification faster is the calculation of the linear relationship between the gray value and the automatically counted number of conidia that has only to be performed once in the beginning of analysis. Afterwards, the gray value is used as single parameter for quantification. The fast, easy and objective determination of sporulation capacity enables facilitated quality control of fungal formulations designed for biological pest control.•Rapid, simple, objective and reproducible quantification of fungal sporulation suitable for large-scale sample quantities.•Requires conventional laboratory equipment and open-source software without technical or computational expertise.•The number of automatically counted conidia can be correlated with the gray value and after initial calculation of a linear fit, the gray value can be applied as single quantification parameter. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Authors.)
Databáze:	MEDLINE
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