| Autor: |
Brovkina OI; Research Institute of General Pathology and Pathophysiology, Moscow, 125315 Russia.; Federal Scientific and Clinical Center for Specialized Types of Medical Aid and Medical Technologies of FMBA of Russia, Moscow, 115682 Russia.; brov.olia@gmail.com., Pronina IV; Research Institute of General Pathology and Pathophysiology, Moscow, 125315 Russia., Uroshlev LA; Research Institute of General Pathology and Pathophysiology, Moscow, 125315 Russia.; Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 117971 Russia., Fridman MV; Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 117971 Russia., Loginov VI; Research Institute of General Pathology and Pathophysiology, Moscow, 125315 Russia., Kazubskaya TP; Blokhin National Medical Research Center of Oncology of the Ministry of Health, Russian Federation, Moscow, 115478 Russia., Utkin DO; Blokhin National Medical Research Center of Oncology of the Ministry of Health, Russian Federation, Moscow, 115478 Russia., Kushlinskii NE; Blokhin National Medical Research Center of Oncology of the Ministry of Health, Russian Federation, Moscow, 115478 Russia., Braga EA; Research Institute of General Pathology and Pathophysiology, Moscow, 125315 Russia. |
| Abstrakt: |
Recently, a wealth of data have been accumulating on the role of long non-coding RNAs (lncRNAs) in the fine-tuning of mRNA expression. Four new lncRNAs, namely, TMEM92-AS1, FAM222A-AS, TXLNB, and lnc-CCL28, were identified as differentially expressed in ovarian tumors using deep machine learning. The levels of lnc-CCL28 transcripts in both tumors and normal tissue samples were sufficient for further analysis by RT-PCR. In addition, the promising ovarian cancer biomarkers, lncRNAs LINC00152, NEAT 1 and SNHG17 were added to RT-PCR analysis. For the first time, an increase in the level of lnc-CCL28 and SNHG 17 lncRNAs was found in ovarian tumors, and the overexpression of LINC00152 and NEAT1 was confirmed. It seems that lnc-CCL28 is involved in carcinogenesis and, in particular, in ovarian cancer progression. Overexpression of LINC00152 and lnc-CCL28 was significantly associated with the later stages and metastasis. |
