Rapid Screening of COVID-19 Directly from Clinical Nasopharyngeal Swabs Using the MasSpec Pen.

Autor:	Garza KY; Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States., Silva AAR; MS4Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University, Bragança Paulista, São Paulo 12916-900, Brazil., Rosa JR; MS4Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University, Bragança Paulista, São Paulo 12916-900, Brazil., Keating MF; Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States., Povilaitis SC; Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States., Spradlin M; Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States., Sanches PHG; MS4Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University, Bragança Paulista, São Paulo 12916-900, Brazil., Varão Moura A; MS4Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University, Bragança Paulista, São Paulo 12916-900, Brazil., Marrero Gutierrez J; MS4Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University, Bragança Paulista, São Paulo 12916-900, Brazil., Lin JQ; Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States., Zhang J; Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States., DeHoog RJ; Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States., Bensussan A; Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States., Badal S; Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States., Cardoso de Oliveira D; MS4Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University, Bragança Paulista, São Paulo 12916-900, Brazil., Dias Garcia PH; MS4Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University, Bragança Paulista, São Paulo 12916-900, Brazil., Dias de Oliveira Negrini L; Municipal Department of Health, Bragança Paulista, Sao Paulo 12902-230, Brazil., Antonio MA; Integrated Unit of Pharmacology and Gastroenterology, UNIFAG, Bragança Paulista, Sao Paulo 12916-900, Brazil., Canevari TC; School of Material Engineering and Nanotechnology, MackMass Laboratory, Mackenzie Presbyterian University, São Paulo, SP 01302-907, Brazil., Eberlin MN; School of Material Engineering and Nanotechnology, MackMass Laboratory, Mackenzie Presbyterian University, São Paulo, SP 01302-907, Brazil., Tibshirani R; Department of Biomedical Data Science, Stanford University, Stanford, California 94305, United States., Eberlin LS; Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States., Porcari AM; MS4Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University, Bragança Paulista, São Paulo 12916-900, Brazil.
Jazyk:	angličtina
Zdroj:	Analytical chemistry [Anal Chem] 2021 Sep 21; Vol. 93 (37), pp. 12582-12593. Date of Electronic Publication: 2021 Aug 25.
DOI:	10.1021/acs.analchem.1c01937
Abstrakt:	The outbreak of COVID-19 has created an unprecedent global crisis. While the polymerase chain reaction (PCR) is the gold standard method for detecting active SARS-CoV-2 infection, alternative high-throughput diagnostic tests are of a significant value to meet universal testing demands. Here, we describe a new design of the MasSpec Pen technology integrated to electrospray ionization (ESI) for direct analysis of clinical swabs and investigate its use for COVID-19 screening. The redesigned MasSpec Pen system incorporates a disposable sampling device refined for uniform and efficient analysis of swab tips via liquid extraction directly coupled to an ESI source. Using this system, we analyzed nasopharyngeal swabs from 244 individuals including symptomatic COVID-19 positive, symptomatic negative, and asymptomatic negative individuals, enabling rapid detection of rich lipid profiles. Two statistical classifiers were generated based on the lipid information acquired. Classifier 1 was built to distinguish symptomatic PCR-positive from asymptomatic PCR-negative individuals, yielding a cross-validation accuracy of 83.5%, sensitivity of 76.6%, and specificity of 86.6%, and validation set accuracy of 89.6%, sensitivity of 100%, and specificity of 85.3%. Classifier 2 was built to distinguish symptomatic PCR-positive patients from negative individuals including symptomatic PCR-negative patients with moderate to severe symptoms and asymptomatic individuals, yielding a cross-validation accuracy of 78.4%, specificity of 77.21%, and sensitivity of 81.8%. Collectively, this study suggests that the lipid profiles detected directly from nasopharyngeal swabs using MasSpec Pen-ESI mass spectrometry (MS) allow fast (under a minute) screening of the COVID-19 disease using minimal operating steps and no specialized reagents, thus representing a promising alternative high-throughput method for screening of COVID-19.
Databáze:	MEDLINE
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