| Autor: |
Ashraf AA; Department of Medical Physiology, Faculty of Medicine, Cairo University, Giza, Egypt., Gamal SM; Department of Medical Physiology, Faculty of Medicine, Cairo University, Giza, Egypt., Ashour H; Department of Medical Physiology, Faculty of Medicine, Cairo University, Giza, Egypt.; Department of Medical Physiology, Faculty of Medicine, King Khalid University, Abha, Kingdom of Saudi Arabia., Aboulhoda BE; Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Giza, Egypt., Rashed LA; Department of Biochemistry, Faculty of Medicine, Cairo University, Giza, Egypt., Harb IA; Department of Pharmacology, Faculty of Medicine, Cairo University, Giza, Egypt., Abdelfattah GH; Department of Anatomy and Embryology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt., El-Seidi EA; Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Giza, Egypt., Shawky HM; Department of Medical Physiology, Faculty of Medicine, Cairo University, Giza, Egypt. |
| Abstrakt: |
Multiple theories have been proposed describing the pathogenic mechanisms of Helicobacter pylori ( H. pylori )-associated gastric motility disorders. We assessed ex vivo pyloric activity in H. pylori -infected rats, and tried to explore the associated ghrelin hormone alteration and pyloric fibrogenesis. In addition, miR-1 was assessed in pyloric tissue samples, being recently accused of having a role in smooth muscle dysfunction. Ninety adult male Wistar albino rats were assigned into nine groups: 1 ) control group, 2 ) sterile broth (vehicle group), 3 ) amoxicillin control, 4 ) omeperazole control, 5 ) clarithromycin control, 6 ) triple therapy control, 7 ) H. pylori - group, 8 ) H. pylori -clarithromycin group, and 9 ) H. pylori -triple therapy group. Urease enzyme activity was applied as an indicator of H. pylori infection. Ex vivo pyloric contractility was evaluated. Serum ghrelin was assessed, and histological tissue evaluation was performed. Besides, pyloric muscle miR-1 expression was measured. The immunological epithelial to mesenchymal transition (EMT) markers; transforming growth factor β (TGFβ), α-smooth muscle actin (α-SMA), and E-cadherin-3 were also evaluated. By H. pylori infection, a significant ( P < 0.001) reduced pyloric contractility index was recorded. The miR-1 expression was decreased ( P < 0.001) in the H. pylori- infected group, associated with reduced serum ghrelin, elevated TGFβ, and α-SMA levels and reduced E-cadherin levels. Decreased miR-1 and disturbed molecular pattern were improved by treatment. In conclusion, H. pylori infection was associated with reduced miR-1, epithelial to mesenchymal transition, and pyloric hypomotility. The miR-1 may be a target for further studies to assess its possible involvement in H. pylori -associated pyloric dysfunction, which might help in the management of human H. pylori manifestations and complications. NEW & NOTEWORTHY This work is investigating functional, histopathological, and molecular changes underlying Helicobacter pylori hypomotility and is correlating these with miR-1, whose disturbance is supposed to be involved in smooth muscle dysfunction and cell proliferation according to literature. Epithelial to mesenchymal transition and reduced ghrelin hormone may contribute to H. pylori infection-associated hypomotility. H. pylori infection was associated with reduced pyloric miR-1 expression. Targeting miR-1 could be valuable in the clinical management of pyloric hypofunction. |
