Graphene nanofiber composites for enhanced neuronal differentiation of human mesenchymal stem cells.

Autor:	Rawat S; Stem Cell Facility, DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi, 110029, India., Jain KG; Stem Cell Facility, DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi, 110029, India., Gupta D; SMITA Research Lab, Department of Textile & Fibre Engineering, Indian Institute of Technology, New Delhi, 110016, India., Raghav PK; Stem Cell Facility, DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi, 110029, India., Chaudhuri R; Stem Cell Facility, DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi, 110029, India., Pinky; Stem Cell Facility, DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi, 110029, India., Shakeel A; Stem Cell Facility, DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi, 110029, India., Arora V; SMITA Research Lab, Department of Textile & Fibre Engineering, Indian Institute of Technology, New Delhi, 110016, India., Sharma H; Stem Cell Facility, DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi, 110029, India., Debnath D; Department of Biomedical Engineering, Department of Mechanical Engineering, University of Bridgeport, Bridgeport, CT 06604, USA., Kalluri A; Department of Biomedical Engineering, Department of Mechanical Engineering, University of Bridgeport, Bridgeport, CT 06604, USA., Agrawal AK; SMITA Research Lab, Department of Textile & Fibre Engineering, Indian Institute of Technology, New Delhi, 110016, India., Jassal M; SMITA Research Lab, Department of Textile & Fibre Engineering, Indian Institute of Technology, New Delhi, 110016, India., Dinda AK; Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India., Patra P; Department of Biomedical Engineering, Department of Mechanical Engineering, University of Bridgeport, Bridgeport, CT 06604, USA., Mohanty S; Stem Cell Facility, DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi, 110029, India.
Jazyk:	angličtina
Zdroj:	Nanomedicine (London, England) [Nanomedicine (Lond)] 2021 Sep; Vol. 16 (22), pp. 1963-1982. Date of Electronic Publication: 2021 Aug 25.
DOI:	10.2217/nnm-2021-0121
Abstrakt:	Aim: To differentiate mesenchymal stem cells into functional dopaminergic neurons using an electrospun polycaprolactone (PCL) and graphene (G) nanocomposite. Methods: A one-step approach was used to electrospin the PCL nanocomposite, with varying G concentrations, followed by evaluating their biocompatibility and neuronal differentiation. Results: PCL with exiguous graphene demonstrated an ideal nanotopography with an unprecedented combination of guidance stimuli and substrate cues, aiding the enhanced differentiation of mesenchymal stem cells into dopaminergic neurons. These newly differentiated neurons were seen to exhibit unique neuronal arborization, enhanced intracellular Ca 2+ influx and dopamine secretion. Conclusion: Having cost-effective fabrication and room-temperature storage, the PCL-G nanocomposites could pave the way for enhanced neuronal differentiation, thereby opening a new horizon for an array of applications in neural regenerative medicine.
Databáze:	MEDLINE
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