A bioengineering method for modeling alveolar Rhabdomyosarcoma and assessing chemotherapy responses.

Autor:	Stefanek E; Laboratory for Innovations in Micro Engineering (LiME), Department of Mechanical Engineering, University of Victoria, Victoria, Canada.; Centre for Advanced Materials and Related Technologies (CAMTEC), University of Victoria, Victoria, BC V8P 5C2, Canada., Samiei E; Laboratory for Innovations in Micro Engineering (LiME), Department of Mechanical Engineering, University of Victoria, Victoria, Canada., Kavoosi M; Department of Biology, School of Basic Sciences, Research and Science Branch of Islamic Azad University, Zanjan, Iran., Esmaeillou M; Department of Medical Biotechnologies, University of Siena, Siena, Italy., Roustai Geraylow K; Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran., Emami A; Faculty of Psychology, Department of Health, York University, ON, Canada.; Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada., Ashrafizadeh M; Faculty of Engineering and Natural Sciences, Sabanci University, Orta Mahalle, Üniversite Caddesi No. 27, Orhanlı, Tuzla, Istanbul 34956, Turkey., Perrin D; Department of Surgery, Section of Orthopaedic Surgery, University of Manitoba, Winnipeg MB R3A 1R9, Canada., Gordon JW; Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.; The Diabetes Research Envisioned and Accomplished in Manitoba (DREAM) Theme of the Children's Hospital Research Institute of Manitoba, Canada., Akbari M; Laboratory for Innovations in Micro Engineering (LiME), Department of Mechanical Engineering, University of Victoria, Victoria, Canada.; Centre for Advanced Materials and Related Technologies (CAMTEC), University of Victoria, Victoria, BC V8P 5C2, Canada.; Biotechnology Center, Silesian University of Technology, Akademicka 2A, Gliwice 44-100, Poland., Ghavami S; Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.; Research Institute of Oncology and Hematology, Cancer Care Manitoba, University of Manitoba, Winnipeg MBR3E 0V9, Canada.; Autophagy Research Center, Shiraz University of Medical Sciences, Shiraz 7134845794, Iran.; Faculty of Medicine, Katowice School of Technology, Katowice, Poland.
Jazyk:	angličtina
Zdroj:	MethodsX [MethodsX] 2021 Jul 27; Vol. 8, pp. 101473. Date of Electronic Publication: 2021 Jul 27 (Print Publication: 2021).
DOI:	10.1016/j.mex.2021.101473
Abstrakt:	Rhabdomyosarcoma (RMS) is the most common pediatric soft-tissue malignant tumor. Treatment of RMS usually includes primary tumor resection along with systemic chemotherapy. Two-dimensional (2D) cell culture systems and animal models have been extensively used for investigating the potential efficacy of new RMS treatments. However, RMS cells behave differently in 2D culture than in vivo, which has recently inspired the adoption of three-dimensional (3D) culture environments. In the current paper, we will describe the detailed methodology we have developed for fabricating a 3D engineered model to study alveolar RMS (ARMS) in vitro. This model consists of a thermally cross-linked collagen disk laden with RMS cells that mimics the structural and bio-chemical aspects of the tumor extracellular matrix (ECM). This process is highly reproducible and produces a 3D engineered model that can be used to analyze the cytotoxicity and autophagy induction of drugs on ARMS cells. The most improtant bullet points are as following:•We fabricated 3D model of ARMS.•The current ARMS 3D model can be used for screening of chemotherapy drugs.•We developed methods to detect apoptosis and autophagy in ARMS 3D model to detect the mechansims of chemotherapy agents. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Authors.)
Databáze:	MEDLINE
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