Sperm selection of cryopreserved milt of catfish ( Rhamdia quelen ) by density gradient centrifugation with AllGrad® 90.

Autor:	Pérez-Atehortúa M; Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil., Galuppo A; Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil., Rodrigues RB; Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil., Teixeira NDS; Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil., de Freitas TR; Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil., França TS; Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil., Marques LS; Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil., Gomes IC; Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil., Streit DP Jr; Animal Science Research Program of Federal University of Rio Grande do Sul, Bento Gonçalves, 7712, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil.
Jazyk:	angličtina
Zdroj:	MethodsX [MethodsX] 2021 Jun 19; Vol. 8, pp. 101422. Date of Electronic Publication: 2021 Jun 19 (Print Publication: 2021).
DOI:	10.1016/j.mex.2021.101422
Abstrakt:	Density gradient centrifugation is a technique used to wash or separate samples of cryopreserved milt, mainly in humans and bovines allowing, for example, reducing the concentration of cryoprotectants or choosing the best portion of sperm. The proposed method seeks to reduce the presence of cryoprotectant in the cryopreserved milt of the Rhamdia qhelen and to obtain a fraction of better quality sperm. Gradient centrifugation was formed from 90% AllGrad® and different centrifugation times and forces were compared. The separated sperm presented a low increase in motility and decreased head damage and presence of gout, however, it was better compared to the non-separated samples. The speed of 1000 × g for 10 min, 4 °C, allowed 22.25 ± 4.64% of normal spermatozoa, that is, 9.25% more than the non-centrifuged milt ( p  = 0.0013).•The centrifugation method allows a fraction of spermatozoa morphologically less affected by cryopreservation.•Density gradient centrifugation with AllGrad® 90% is proposed as a tool of easy adaptation and application for the separation of cryopreserved sperm of R. quelen .•Density gradient centrifugation method at 1000 × g for 10 min allows obtaining a better fraction of normal sperm. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Author(s). Published by Elsevier B.V.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu