Preliminary Histological Evaluation of the Application of Ozone in the First Days of Orthodontic Force Induction in Animal Model.
| Autor: | Faccini M; Department of Orthodontics, Ingá University Center UNINGA, Maringá, Brazil., Agostini F; Department of Orthodontics, Ingá University Center UNINGA, Maringá, Brazil., Drieu T; Department of Dentistry, São Leopoldo Mandic, Campinas, Brazil., Campos FUF; Department of Oral Microbiology, São Leopoldo Mandic, Campinas, Brazil., Garcez A; Department of Oral Microbiology, São Leopoldo Mandic, Campinas, Brazil., Carinhena GF; Department of Orthodontics, Superior Dentistry School, Brazil., Salmeron S; Department of Periodontics, Ingá University Center UNINGA, Maringá, Brazil., Casaroto AR; Department of Pathology, Ingá University Center UNINGA, Maringá, Brazil., Valarelli FP; Department of Orthodontics, Ingá University Center UNINGA, Maringá, Brazil., Freitas KMS; Department of Orthodontics, Ingá University Center UNINGA, Maringá, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | European journal of dentistry [Eur J Dent] 2022 Feb; Vol. 16 (1), pp. 122-129. Date of Electronic Publication: 2021 Aug 24. |
| DOI: | 10.1055/s-0041-1731886 |
| Abstrakt: | Objectives: The aim of the study was to histologically evaluate the effect of ozone therapy on orthodontic force induction in an animal model. Materials and Methods: Twenty-four Wistar rats were divided into three groups ( n = 8). A NiTi coil spring was installed from the maxillary first molar to the maxillary central incisor. G1 was control and G2/G3 received 1 mL of ozonated gas at concentrations of 10 and 60 µg/mL, in the buccal mucosa above the first molar roots. The animals were euthanized 3 and 5 days after the procedure. Histological sections were obtained, longitudinally of the first molar' long axis, in the mesiodistal direction. The number of osteoclasts, osteoblasts, blood vessels, polymorphonuclear and mononuclear cells, formation of osteoid tissue and hyaline areas, and root resorption were evaluated with light microscope, in tension and pressure sides. Intergroup comparisons were performed with Kruskal-Wallis, Dunn, and Chi-square tests. Results: At 3-days pressure side, a greater number of osteoclasts was observed in ozone groups and greater number of blood vessels and polymorphonuclear cells were observed in G2. On the tension side, there was a significantly greater number of blood vessels, osteoblasts, and mononuclear cells in G2. At 5-days pressure side, there was a significantly greater number of osteoclasts in G2, blood vessels and osteoblasts in the ozone groups, and lesser number of polymorphonuclear cells in G3. Conclusion: Ozone therapy increased the number of osteoclasts on the pressure side and osteoblasts on tension side, in 10 µg/mL concentration, demonstrating histological parameters favorable to bone remodeling. The 60 µg/mL ozone concentration accelerated the periodontal ligament reorganization process. Competing Interests: None declared. (The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/).) |
| Databáze: | MEDLINE |
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