Acute SARS-CoV-2 infections harbor limited within-host diversity and transmit via tight transmission bottlenecks.

Autor: Braun KM; Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Moreno GK; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Wagner C; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America., Accola MA; University of Wisconsin School of Medicine and Public Health and the William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin, United States of America., Rehrauer WM; University of Wisconsin School of Medicine and Public Health and the William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin, United States of America., Baker DA; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Koelle K; Department of Biology, Emory University, Atlanta, Georgia, United States of America., O'Connor DH; Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.; Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Bedford T; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America., Friedrich TC; Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Moncla LH; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2021 Aug 23; Vol. 17 (8), pp. e1009849. Date of Electronic Publication: 2021 Aug 23 (Print Publication: 2021).
DOI: 10.1371/journal.ppat.1009849
Abstrakt: The emergence of divergent SARS-CoV-2 lineages has raised concern that novel variants eliciting immune escape or the ability to displace circulating lineages could emerge within individual hosts. Though growing evidence suggests that novel variants arise during prolonged infections, most infections are acute. Understanding how efficiently variants emerge and transmit among acutely-infected hosts is therefore critical for predicting the pace of long-term SARS-CoV-2 evolution. To characterize how within-host diversity is generated and propagated, we combine extensive laboratory and bioinformatic controls with metrics of within- and between-host diversity to 133 SARS-CoV-2 genomes from acutely-infected individuals. We find that within-host diversity is low and transmission bottlenecks are narrow, with very few viruses founding most infections. Within-host variants are rarely transmitted, even among individuals within the same household, and are rarely detected along phylogenetically linked infections in the broader community. These findings suggest that most variation generated within-host is lost during transmission.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje