Investigation of anthelmintic activity of the acetone extract and constituents of Typha capensis against animal parasitic Haemonchus contortus and free-living Caenorhabditis elegans.

Autor: Ondua M; Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa.; School of Veterinary Medicine and Science, University of Ngaoundéré, P.O. BOX 454, Ngaoundéré, Cameroon., Mfotie Njoya E; Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa., Abdalla MA; Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa.; Department of Food Science and Technology, Faculty of Agriculture, University of Khartoum, 13314, Khartoum North, Sudan.; Institut Für Pflanzenernährung Und Bodenkunde, Christian-Albrechts-Universität Zu Kiel, Hermann-Rodewald-Strasse 2, 24118, Kiel, Germany., McGaw LJ; Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa. lyndy.mcgaw@up.ac.za.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2021 Oct; Vol. 120 (10), pp. 3437-3449. Date of Electronic Publication: 2021 Aug 23.
DOI: 10.1007/s00436-021-07269-7
Abstrakt: This study aimed to determine in vitro anthelmintic activity of plant extracts of eleven plant species used traditionally in South Africa to treat various disorders including symptoms related to nematode infections, and to isolate bioactive compounds from the most active plant extract. Crude plant extracts were tested on different life-cycle stages of Haemonchus contortus. The cytotoxicity of the most active extracts, fractions and compounds was evaluated on Vero cells and the most potent extract, fractions and compounds were tested for their ability to kill the parasitic H. contortus and the free-living nematode Caenorhabditis elegans. Typha capensis acetone extract had the strongest egg hatching inhibitory effect with an EC 50 of 184.94 μg/mL, and this extract also halted larval development of H. contortus with an EC 50 of 83.30 μg/mL compared to the positive control (albendazole) with an EC 50 of 2.66 μg/mL. Typha capensis crude extract and its butanol fraction had promising anthelmintic activity against both parasitic H. contortus and free-living C. elegans. Two compounds isolated from T. capensis, namely, isorhamnetin-3-O-β-D-glucoside and isorhamnetin 3-O-rutinoside, had antioxidant activity with IC 50 values of 3.16 μg/mL and 0.96 μg/mL respectively, and good anthelmintic activity against H. contortus with IC 50 values of 55.61 μg/mL and 145.17 μg/mL respectively. Identification of bioactive compounds from the T. capensis crude extract supports development of this extract as a complementary or alternative treatment against haemonchosis. However, further research is necessary to confirm the anthelmintic efficacy of the plant, including in vivo studies.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: