Integrated single-cell analysis unveils diverging immune features of COVID-19, influenza, and other community-acquired pneumonia.

Autor:	Schuurman AR; Center for Experimental and Molecular Medicine, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.; Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Amsterdam, Netherlands., Reijnders TDY; Center for Experimental and Molecular Medicine, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.; Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Amsterdam, Netherlands., Saris A; Center for Experimental and Molecular Medicine, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.; Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Amsterdam, Netherlands., Ramirez Moral I; Center for Experimental and Molecular Medicine, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.; Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Amsterdam, Netherlands., Schinkel M; Center for Experimental and Molecular Medicine, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.; Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Amsterdam, Netherlands., de Brabander J; Center for Experimental and Molecular Medicine, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.; Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Amsterdam, Netherlands., van Linge C; Center for Experimental and Molecular Medicine, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.; Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Amsterdam, Netherlands., Vermeulen L; Laboratory for Experimental Oncology and Radiobiology, Center for Experimental and Molecular Medicine, Cancer Center Amsterdam and Amsterdam Gastroenterology and Metabolism, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands., Scicluna BP; Center for Experimental and Molecular Medicine, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.; Division of Infectious Diseases, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands., Wiersinga WJ; Center for Experimental and Molecular Medicine, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.; Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Amsterdam, Netherlands.; Department of Clinical Epidemiology, Biostatistics and Bioinformatics, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands., Vieira Braga FA; Laboratory for Experimental Oncology and Radiobiology, Center for Experimental and Molecular Medicine, Cancer Center Amsterdam and Amsterdam Gastroenterology and Metabolism, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands., van der Poll T; Center for Experimental and Molecular Medicine, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.; Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Amsterdam, Netherlands.; Department of Clinical Epidemiology, Biostatistics and Bioinformatics, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 Aug 23; Vol. 10. Date of Electronic Publication: 2021 Aug 23.
DOI:	10.7554/eLife.69661
Abstrakt:	The exact immunopathophysiology of community-acquired pneumonia (CAP) caused by SARS-CoV-2 (COVID-19) remains clouded by a general lack of relevant disease controls. The scarcity of single-cell investigations in the broader population of patients with CAP renders it difficult to distinguish immune features unique to COVID-19 from the common characteristics of a dysregulated host response to pneumonia. We performed integrated single-cell transcriptomic and proteomic analyses in peripheral blood mononuclear cells from a matched cohort of eight patients with COVID-19, eight patients with CAP caused by Influenza A or other pathogens, and four non-infectious control subjects. Using this balanced, multi-omics approach, we describe shared and diverging transcriptional and phenotypic patterns-including increased levels of type I interferon-stimulated natural killer cells in COVID-19, cytotoxic CD8 T EMRA cells in both COVID-19 and influenza, and distinctive monocyte compositions between all groups-and thereby expand our understanding of the peripheral immune response in different etiologies of pneumonia. Competing Interests: AS, TR, AS, IR, MS, Jd, Cv, LV, BS, WW, FV, Tv No competing interests declared (© 2021, Schuurman et al.)
Databáze:	MEDLINE
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