In Vitro Evaluation of the Toxicological Profile and Oxidative Stress of Relevant Diet-Related Advanced Glycation End Products and Related 1,2-Dicarbonyls.

Autor: Cepas V; Departamento de Morfología y Biología Celular, Universidad de Oviedo, Spain.; Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, Instituto de investigación Sanitaria del Principado de Asturias (ISPA), Spain., Manig F; Chair of Food Chemistry, Technische Universität Dresden, D-01062 Dresden, Germany., Mayo JC; Departamento de Morfología y Biología Celular, Universidad de Oviedo, Spain.; Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, Instituto de investigación Sanitaria del Principado de Asturias (ISPA), Spain., Hellwig M; Chair of Food Chemistry, Technische Universität Dresden, D-01062 Dresden, Germany.; Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstraße 20, D-38106 Braunschweig, Germany., Collotta D; Department of Neurosciences 'Rita Levi Montalcini', University of Turin, Italy., Sanmartino V; Department of Neurosciences 'Rita Levi Montalcini', University of Turin, Italy., Carrocera-Pumarino R; Departamento de Morfología y Biología Celular, Universidad de Oviedo, Spain.; Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, Instituto de investigación Sanitaria del Principado de Asturias (ISPA), Spain., Collino M; Department of Neurosciences 'Rita Levi Montalcini', University of Turin, Italy., Henle T; Chair of Food Chemistry, Technische Universität Dresden, D-01062 Dresden, Germany., Sainz RM; Departamento de Morfología y Biología Celular, Universidad de Oviedo, Spain.; Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, Instituto de investigación Sanitaria del Principado de Asturias (ISPA), Spain.
Jazyk: angličtina
Zdroj: Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2021 Aug 08; Vol. 2021, pp. 9912240. Date of Electronic Publication: 2021 Aug 08 (Print Publication: 2021).
DOI: 10.1155/2021/9912240
Abstrakt: During food processing and storage, and in tissues and fluids under physiological conditions, the Maillard reaction occurs. During this reaction, reactive 1,2-dicarbonyl compounds arise as intermediates that undergo further reactions to form advanced glycation end products (AGEs). Diet is the primary source of exogenous AGEs. Endogenously formed AGEs have been proposed as a risk factor in the pathogenesis of diet-related diseases such as diabetes, insulin resistance, cardiovascular diseases, or chronic disease. AGEs may differently contribute to the diet-related exacerbation of oxidative stress, inflammation, and protein modifications. Here, to understand the contribution of each compound, we tested individually, for the first time, the effect of five 1,2-dicarbonyl compounds 3-deoxyglucosone (3-DG), 3-deoxygalactosone (3-DGal), 3,4-dideoxyglucosone-3-ene (3,4-DGE), glyoxal (GO), and methylglyoxal (MGO) and four different glycated amino acids N- ε -(carboxyethyl)lysine (CEL), N- ε -(carboxymethyl)lysine (CML), methylglyoxal-derived hydroimidazolone-1 (MG-H1), and pyrraline (Pyrr) in a cell line of human keratinocytes (HaCaT). We found that most of the glycated amino acids, i.e., CEL, CML, and MG-H1, did not show any cytotoxicity. At the same time, 1,2-dicarbonyl compounds 3-DGal, 3,4-DGE, GO, and MGO increased the production of reactive oxygen species and induced cell death. MGO induced cell death by apoptosis, whereas 3-DGal and 3,4-DGE induced nuclear translocation of the proinflammatory NF- κ B transcription pathway, and the activation of the pyroptosis-related NLRP3 inflammasome cascade. Overall, these results demonstrate the higher toxic impact of 1,2-dicarbonyl compounds on mucosal epithelial cells when compared to glycated amino acids and the selective activation of intracellular signaling pathways involved in the crosstalk mechanisms linking oxidative stress to excessive inflammation.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2021 Vanesa Cepas et al.)
Databáze: MEDLINE
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