Use of MALDI-TOF mass spectrometry to explore the peptidome and proteome of in-vitro produced bovine embryos pre-exposed to oviduct fluid.

Autor: Banliat C; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380, Nouzilly, France; Union Evolution, Noyal-Sur-Vilaine, France., Labas V; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380, Nouzilly, France; INRAE, Université de Tours, CHU de Tours, PIXANIM, 37380 Nouzilly, France., Tomas D; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380, Nouzilly, France; INRAE, Université de Tours, CHU de Tours, PIXANIM, 37380 Nouzilly, France., Teixeira-Gomes AP; INRAE, Université de Tours, CHU de Tours, PIXANIM, 37380 Nouzilly, France; INRAE, Université de Tours, ISP, 37380 Nouzilly, France., Guyonnet B; Union Evolution, Noyal-Sur-Vilaine, France., Mermillod P; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380, Nouzilly, France., Saint-Dizier M; CNRS, IFCE, INRAE, Université de Tours, PRC, 37380, Nouzilly, France; Tours University, Tours, France. Electronic address: marie.saint-dizier@univ-tours.fr.
Jazyk: angličtina
Zdroj: Reproductive biology [Reprod Biol] 2021 Dec; Vol. 21 (4), pp. 100545. Date of Electronic Publication: 2021 Aug 19.
DOI: 10.1016/j.repbio.2021.100545
Abstrakt: In order to identify oviduct fluid (OF) peptides and proteins possibly uptaken by developing embryos, in-vitro produced bovine embryos exposed or not to OF were individually analyzed by MALDI-TOF mass spectrometry. Overall, 11 masses were overabundant in OF-treated embryos compared to controls, among which one at 8.9 kDa annotated as immediate early response 3-interacting protein 1 or a peptide of transitional endoplasmic reticulum ATPase met the criteria of an OF embryo-interacting protein or peptide.
Competing Interests: Declaration of Competing Interest The authors report no declarations of interest.
(Copyright © 2021 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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