Toxic effect of Croton rudolphianus leaf essential oil against Biomphalaria glabrata, Schistosoma mansoni cercariae and Artemia salina.

Autor: Ribeiro IATA; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil. Electronic address: ingridd.torres@hotmail.com., Sá JLF; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Lima MV; Departamento de Biofísica e Radiobiologia, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Veras STS; Departamento de Engenharia Civil e Ambiental, Centro de Tecnologia e Geociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Aguiar JCROF; Departamento de Química Fundamental, Centro de Ciências Exatas e da Natureza, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Aires AL; Departamento de Medicina Tropical, Centro de Ciência da Saúde, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Albuquerque MCPA; Departamento de Medicina Tropical, Centro de Ciência da Saúde, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., da Silva MV; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Melo AMMA; Departamento de Biofísica e Radiobiologia, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Navarro DMAF; Departamento de Química Fundamental, Centro de Ciências Exatas e da Natureza, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Correia MTS; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2021 Nov; Vol. 223, pp. 106102. Date of Electronic Publication: 2021 Aug 17.
DOI: 10.1016/j.actatropica.2021.106102
Abstrakt: This research investigated the effect of the Croton rudolphianus leaf essential oil (EO) on Biomphalaria glabrata embryos (at different development stages) and adults, Schistosoma mansoni cercariae, and Artemia salina (non-target organism). It was possible to identify 31 compounds in the C. rudolphianus EO through GC-MS analysis. The major compounds from this oil were (E)-caryophyllene (17.33%), an unknown compound (16.87%), bicyclogermacrene (7.1%), δ-cadinene (6.62%) and germacrene D (5.38%). After incubation for 24 h, the EO of C. rudolphianus induced the occurrence of non-viable embryos (dead and malformed), with an LC 50 value of 126.54, 133.51, 143.53 and 161.95 µg/mL and an LC 90 value of 202.61, 216.48, 232.98 and 271.16 µg/mL to blastula, gastrula, trochophore and veliger embryonic stages, respectively. The EO was more effective against B. glabrata adults (LC 50 and LC 90  = 47.89 and 78.86 µg/mL, respectively), and S. mansoni cercariae (LC 50 and LC 90  = 14.81 and 22.15 after 120 mins of exposure, respectively) than against B. glabrata embryos. Concerning the micronucleus assay, the mean frequency of apoptosis, binucleation and micronucleus were 45.33 ± 3.51, 19.33 ± 1.53 and 0.67 ± 0.58 per 1000 cells at 25 µg/mL, which is the highest concentration tested. The oil killed A. salina with LC 50 and LC 90 values (68.33 and 111.5 µg/mL, respectively) higher than those determined for adult snails and S. mansoni cercariae. In conclusion, C. rudolphianus EO had a toxic effect against B. glabrata adults and embryos, and S. mansoni cercariae. Furthermore, this oil showed to be cytotoxic to hemocytes of B. glabrata. Concerning the non-target organism assay, C. rudolphianus EO was less toxic to A. salina then to adult snails and S. mansoni cercariae. Due to this, the EO from C. rudolphianus leaves is a potential alternative for schistosomiasis control.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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