Neutrophils dominate in opsonic phagocytosis of P. falciparum blood-stage merozoites and protect against febrile malaria.

Autor: Garcia-Senosiain A; Department for Congenital Disorders, Statens Serum Institut, Copenhagen, Denmark.; Centre for Medical Parasitology at Department of International Health, Immunology and Microbiology, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Kana IH; Department for Congenital Disorders, Statens Serum Institut, Copenhagen, Denmark.; Centre for Medical Parasitology at Department of International Health, Immunology and Microbiology, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Singh S; Indian Institute of Integrative Medicine, Jammu, India. subhash0974@gmail.com.; Consytel Life Sciences Pvt. Ltd., Hyderabad, India. subhash0974@gmail.com., Das MK; National Institute of Malaria Research, Field Unit, Ranchi, Jharkhand, India., Dziegiel MH; Blood Bank KI 2034, Department of Clinical Immunology, Copenhagen University Hospital, Copenhagen, Denmark.; Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark., Hertegonne S; Centre for Medical Parasitology at Department of International Health, Immunology and Microbiology, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.; Ghent University, Ghent, Belgium., Adu B; Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Accra, Ghana. BAdu@noguchi.ug.edu.gh., Theisen M; Department for Congenital Disorders, Statens Serum Institut, Copenhagen, Denmark. mth@ssi.dk.; Centre for Medical Parasitology at Department of International Health, Immunology and Microbiology, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark. mth@ssi.dk.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2021 Aug 19; Vol. 4 (1), pp. 984. Date of Electronic Publication: 2021 Aug 19.
DOI: 10.1038/s42003-021-02511-5
Abstrakt: Antibody-mediated opsonic phagocytosis (OP) of Plasmodium falciparum blood-stage merozoites has been associated with protection against malaria. However, the precise contribution of different peripheral blood phagocytes in the OP mechanism remains unknown. Here, we developed an in vitro OP assay using peripheral blood leukocytes that allowed us to quantify the contribution of each phagocytic cell type in the OP of merozoites. We found that CD14  +   + CD16 - monocytes were the dominant phagocytic cells at very low antibody levels and Fc gamma receptor (FcγR) IIA plays a key role. At higher antibody levels however, neutrophils were the main phagocytes in the OP of merozoites with FcγRIIIB acting synergistically with FcγRIIA in the process. We found that OP activity by neutrophils was strongly associated with protection against febrile malaria in longitudinal cohort studies performed in Ghana and India. Our results demonstrate that peripheral blood neutrophils are the main phagocytes of P. falciparum blood-stage merozoites.
(© 2021. The Author(s).)
Databáze: MEDLINE
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