Protocol for in vivo and ex vivo assessments of glucose-stimulated insulin secretion in mouse islet β cells.

Autor:	Zhu YX; Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu 211166, China., Zhou YC; Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu 211166, China., Zhang Y; Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu 211166, China., Sun P; Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu 211166, China., Chang XA; Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu 211166, China., Han X; Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2021 Aug 09; Vol. 2 (3), pp. 100728. Date of Electronic Publication: 2021 Aug 09 (Print Publication: 2021).
DOI:	10.1016/j.xpro.2021.100728
Abstrakt:	Pancreatic islet β cells secrete insulin in a biphasic manner when sensing high blood glucose level. This protocol describes the evaluation of different phases of insulin secretion, as well as basal, glucose-stimulated and total insulin secretion abilities, thereby enabling precise assessment of β cell function both in vivo and ex vivo. The in vivo assay consists of intravenous tube imbedding surgery and hyperglycemic clamp. The ex vivo assay consists of islet isolation, dynamic perfusion and static immersion. For complete details on the use and execution of this protocol, please refer to Sun et al. (2021). Competing Interests: The authors declare no competing interests. (© 2021 The Authors.)
Databáze:	MEDLINE
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