The size and culturability of patient-generated SARS-CoV-2 aerosol.

Autor: Santarpia JL; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA. josh.santarpia@unmc.edu.; Global Center for Health Security, University of Nebraska Medical Center, Omaha, NE, USA. josh.santarpia@unmc.edu.; National Strategic Research Institute, Omaha, NE, USA. josh.santarpia@unmc.edu., Herrera VL; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA.; Global Center for Health Security, University of Nebraska Medical Center, Omaha, NE, USA., Rivera DN; National Strategic Research Institute, Omaha, NE, USA., Ratnesar-Shumate S; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA.; Global Center for Health Security, University of Nebraska Medical Center, Omaha, NE, USA., Reid SP; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA.; Global Center for Health Security, University of Nebraska Medical Center, Omaha, NE, USA., Ackerman DN; National Strategic Research Institute, Omaha, NE, USA., Denton PW; Department of Biology, University of Nebraska Omaha, Omaha, NE, USA., Martens JWS; Department of Biology, University of Nebraska Omaha, Omaha, NE, USA., Fang Y; Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA., Conoan N; Electron Microscopy Core Facility, University of Nebraska Medical Center, Omaha, NE, USA., Callahan MV; Vaccine & Immunotherapy Center, Massachusetts General Hospital, Boston, MA, USA., Lawler JV; Global Center for Health Security, University of Nebraska Medical Center, Omaha, NE, USA.; Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, USA., Brett-Major DM; Global Center for Health Security, University of Nebraska Medical Center, Omaha, NE, USA.; Department of Epidemiology, University of Nebraska Medical Center, Omaha, NE, USA., Lowe JJ; Global Center for Health Security, University of Nebraska Medical Center, Omaha, NE, USA.; Department of Environmental, Agricultural and Occupational Health, University of Nebraska Medical Center, Omaha, NE, USA.
Jazyk: angličtina
Zdroj: Journal of exposure science & environmental epidemiology [J Expo Sci Environ Epidemiol] 2022 Sep; Vol. 32 (5), pp. 706-711. Date of Electronic Publication: 2021 Aug 18.
DOI: 10.1038/s41370-021-00376-8
Abstrakt: Background: Aerosol transmission of COVID-19 is the subject of ongoing policy debate. Characterizing aerosol produced by people with COVID-19 is critical to understanding the role of aerosols in transmission.
Objective: We investigated the presence of virus in size-fractioned aerosols from six COVID-19 patients admitted into mixed acuity wards in April of 2020.
Methods: Size-fractionated aerosol samples and aerosol size distributions were collected from COVID-19 positive patients. Aerosol samples were analyzed for viral RNA, positive samples were cultured in Vero E6 cells. Serial RT-PCR of cells indicated samples where viral replication was likely occurring. Viral presence was also investigated by western blot and transmission electron microscopy (TEM).
Results: SARS-CoV-2 RNA was detected by rRT-PCR in all samples. Three samples confidently indicated the presence of viral replication, all of which were from collected sub-micron aerosol. Western blot indicated the presence of viral proteins in all but one of these samples, and intact virions were observed by TEM in one sample.
Significance: Observations of viral replication in the culture of submicron aerosol samples provides additional evidence that airborne transmission of COVID-19 is possible. These results support the use of efficient respiratory protection in both healthcare and by the public to limit transmission.
(© 2021. The Author(s).)
Databáze: MEDLINE
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