IL-23/IL-17 axis and soluble receptors isoforms sIL-23R and sIL-17RA in patients with rheumatoid arthritis-presenting periodontitis.

Autor: Rodríguez-Montaño R; Instituto de Investigación en Odontología, Departamento de Clínicas Odontológicas Integrales, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, México.; Doctorado en Ciencias Biomédicas (Orientación Inmunología), Centro Universitario de Ciencias de la Salud. Universidad de Guadalajara, Guadalajara, México., Bernard-Medina AG; Servicio de Reumatología, OPD. Hospital Civil de Guadalajara ''Fray Antonio Alcalde'', Guadalajara, México., Oregon-Romero E; Instituto de Investigación en Ciencias Biomédicas, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud. Universidad de Guadalajara, Guadalajara, México., Martínez-Rodríguez VMDC; Especialidad de Periodoncia, Departamento de Clínicas Odontológicas Integrales, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, México., Pita-López ML; Centro de Investigación en Biología Molecular de las Enfermedades Crónicas (CIBIMEC), Departamento de Ciencias Básicas para la Salud, Centro Universitario del Sur. Universidad de Guadalajara, Guadalajara, México., Gómez-Meda BC; Departamento de Biología Molecular y Genómica, Instituto de Genética Humana 'Dr. Enrique Corona Rivera', Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, México., Guerrero-Velázquez C; Instituto de Investigación en Odontología, Departamento de Clínicas Odontológicas Integrales, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, México.
Jazyk: angličtina
Zdroj: Journal of clinical laboratory analysis [J Clin Lab Anal] 2021 Sep; Vol. 35 (9), pp. e23963. Date of Electronic Publication: 2021 Aug 17.
DOI: 10.1002/jcla.23963
Abstrakt: Background: Rheumatoid arthritis (RA) and periodontitis (P) are chronic inflammatory diseases characterized by joint and radiographic bone loss, respectively. IL-23 and IL-17 have an essential role in the immunopathogenesis of RA, and P. IL-23 stimulates Th17 cells through which produces IL-17, IL-21, and RANKL. IL-17 stimulates fibroblasts to produce RANKL, which initiates bone loss in the joints in RA and the periodontal tissue in periodontitis. The aim of this study was to determine the expression pattern of IL-23/IL-17 axis and soluble receptors isoforms sIL-23R and sIL-17RA of patients with RA presenting P (RAP).
Material and Methods: Healthy subjects (HS) (n = 42), patients with P (n = 40), RA (n = 20), and patients with RAP (n = 40) were included. Plasma samples were obtained to evaluate the IL-23, IL-17A, sIL-23R, and sIL-17RA by ELISA technique. A nonparametric Mann-Whitney U test was used to compare the differences between groups. A Chi-square was used to compare gender, grade and stage of periodontitis, and DAS28-ESR between the groups. Spearman's rank correlation coefficient was used to study the association between the molecules and clinical parameters.
Results: IL-23 levels were increased in the RAP group, and lower sIL-23R levels were found in the RAP groups. However, IL-17A was lower in the P and RAP group but not in RA patients. RAP group showed a decrease IL-17A levels in advanced stages of the periodontal disease.
Conclusion: These results suggest that IL-23 and IL-17A tend to downregulate their expression patterns when patients present both rheumatoid arthritis and periodontitis.
(© 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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