Toxicity evaluation of Eleutherine plicata Herb. extracts and possible cell death mechanism.

Autor:	Quadros Gomes AR; Postgraduate Program in Pharmaceutical Innovation, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil., da Rocha Galucio NC; Postgraduate Program in Genetics and Molecular Biology, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil.; Postgraduate Program in Pharmaceutical Sciences, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil., de Albuquerque KCO; Postgraduate Program in Biodiversity and Biotechnology, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil., Brígido HPC; Postgraduate Program in Pharmaceutical Innovation, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil., Varela ELP; Postgraduate Program in Biodiversity and Biotechnology, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil.; Oxidative Stress Research Laboratory, Institute of Biological Sciences, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil., Castro ALG; Postgraduate Program in Genetics and Molecular Biology, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil., Vale VV; Postgraduate Program in Pharmaceutical Innovation, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil., Bahia MO; Postgraduate Program in Neuroscience and Cell Biology, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil., Rodriguez Burbano RM; Postgraduate Program in Genetics and Molecular Biology, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil.; Postgraduate Program in Neuroscience and Cell Biology, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil., de Molfeta FA; Postgraduate Program in Chemistry, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil., Carneiro LA; National Primate Center, Rodovia Br 316, Km. 7, 67.030-000, Ananindeua, PA, Brazil., Percario S; Postgraduate Program in Biodiversity and Biotechnology, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil.; Oxidative Stress Research Laboratory, Institute of Biological Sciences, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil., Dolabela MF; Postgraduate Program in Pharmaceutical Innovation, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil.; Postgraduate Program in Pharmaceutical Sciences, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil.; Postgraduate Program in Biodiversity and Biotechnology, Federal University of Pará, Av. Augusto Corrêa, 1, Guamá, 66075-110, Belém, PA, Brazil.
Jazyk:	angličtina
Zdroj:	Toxicology reports [Toxicol Rep] 2021 Jul 31; Vol. 8, pp. 1480-1487. Date of Electronic Publication: 2021 Jul 31 (Print Publication: 2021).
DOI:	10.1016/j.toxrep.2021.07.015
Abstrakt:	Eleutherine plicata has been shown to be a promising medicinal plant, and its activity has been associated with naphthoquinones. The present study aimed at evaluating the cytotoxicity, genotoxicity, and oral toxicity of the ethanol extract (EEEp), dichloromethane fraction (FDMEp) of E. plicata , and isoeleutherin. For the cytotoxicity evaluation, the viability test (MTT) was used. Genotoxicity was accessed through the Comet assay (alkaline version), acute and subacute oral toxicities were also evaluated. The antioxidant capacity of the samples in the wells where the cells were treated with E. plicata was evaluated. Furthermore, the participation of caspase-8 in the possible mechanism of action of isoeleutherin, eleutherin, and eleutherol was also investigated through a docking study. FDMEp and isoeleutherin were cytotoxic, with higher rates of DNA fragmentation observed for FDMEp and isoeleutherin, and all samples displayed higher antioxidant potential than the control. In the acute oral toxicity test, EEEp, FDMEp, and isoeleutherin did not cause significant clinical changes. In the subacute toxicity assay, EEEp and FDMEp also did not cause clinical, hematological, or biochemical changes. The three compounds bound similarly to caspase-8. Despite the results of cytotoxicity, in vitro studies demonstrated that the use of EEEp appears to be safe and cell death may involve its binding to caspase-8. (© 2021 Published by Elsevier B.V.)
Databáze:	MEDLINE
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