Citral derivative activates cell cycle arrest and apoptosis signaling pathways in Candida albicans by generating oxidative stress.

Autor: Wani MY; University of Jeddah, College of Science, Department of Chemistry, Jeddah 21589, Saudi Arabia. Electronic address: mwani@uj.edu.sa., Ahmad A; Clinical Microbiology and Infectious Diseases, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2193, South Africa; Infection Control, Charlotte Maxeke Johannesburg Academic Hospital, National Health Laboratory Service, Johannesburg 2193, South Africa., Aqlan FM; University of Jeddah, College of Science, Department of Chemistry, Jeddah 21589, Saudi Arabia., Al-Bogami AS; University of Jeddah, College of Science, Department of Chemistry, Jeddah 21589, Saudi Arabia.
Jazyk: angličtina
Zdroj: Bioorganic chemistry [Bioorg Chem] 2021 Oct; Vol. 115, pp. 105260. Date of Electronic Publication: 2021 Aug 10.
DOI: 10.1016/j.bioorg.2021.105260
Abstrakt: For combating life-threatening infections caused by Candida albicans there is an urgent requirement of new antifungal agents with a targeted activity and low host cytotoxicity. Manipulating the mechanistic basis of cell death decision in yeast may provide an alternative approach for future antifungal therapeutics. Herein, the effect of an active citral derivative (Cd1) over the physiology of cell death in C. albicans was assessed. The viability of C. albicans SC5314 cells was determined by broth microdilution assay. The crucial morphological changes and apoptotic markers in Cd1-exposed yeast cells were analyzed. Subsequently the results confirmed that Cd1 arrested growth and caused death in yeast cells. Furthermore, this molecule inhibited antioxidant enzymes that resulted in production of reactive oxygen species. DNA fragmentation and condensation, phosphatidylserine exposure at the outer leaflet of cell membrane, mitochondrial disintegration as well as accumulation of cells at G2/M phase of the cell cycle were recorded. Altogether, this derivative induced apoptotic-type cell death in C. albicans SC5314.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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