In vivo and in vitro 31 P-NMR Study of the Phosphate Transport and Polyphosphate Metabolism in Hebeloma cylindrosporum in Response to Plant Roots Signals.

Autor:	Guernevé CL; INRA, UMR SPO (1083) Sciences pour l'Oenologie, Montpellier, France., Becquer A; INRA, UMR Eco&Sols, Montpellier, France., Torres-Aquino M; INRA, UMR Eco&Sols, Montpellier, France., Amenc LK; INRA, UMR Eco&Sols, Montpellier, France., Trives-Segura C; INRA, UMR Eco&Sols, Montpellier, France., Staunton S; INRA, UMR Eco&Sols, Montpellier, France., Plassard C; INRA, UMR Eco&Sols, Montpellier, France., Quiquampoix H; INRA, UMR Eco&Sols, Montpellier, France.
Jazyk:	angličtina
Zdroj:	Bio-protocol [Bio Protoc] 2018 Aug 20; Vol. 8 (16), pp. e2973. Date of Electronic Publication: 2018 Aug 20 (Print Publication: 2018).
DOI:	10.21769/BioProtoc.2973
Abstrakt:	We used in vivo and in vitro phosphorus-31 nuclear magnetic resonance ( 31 P-NMR) spectroscopy to follow the change in transport, compartmentation and metabolism of phosphate in the ectomycorrhizal fungus Hebeloma cylindrosporum in response to root signals originating from host ( Pinus pinaster ) or non-host ( Zea mays ) plants. A device was developed for the in vivo studies allowing the circulation of a continuously oxygenated mineral solution in an NMR tube containing the mycelia. The in vitro studies were performed on fungal material after several consecutive treatment steps (freezing in liquid nitrogen; crushing with perchloric acid; elimination of perchloric acid; freeze-drying; dissolution in an appropriate liquid medium). Competing Interests: Competing interestsThe authors declare that they have no conflicts of interest. (Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.)
Databáze:	MEDLINE
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