Measuring Secretion of Capsidiol in Leaf Tissues of Nicotiana benthamiana .
| Autor: | Kuroyanagi T; Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan., Camagna M; Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan., Takemoto D; Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan. |
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| Jazyk: | angličtina |
| Zdroj: | Bio-protocol [Bio Protoc] 2018 Aug 05; Vol. 8 (15), pp. e2954. Date of Electronic Publication: 2018 Aug 05 (Print Publication: 2018). |
| DOI: | 10.21769/BioProtoc.2954 |
| Abstrakt: | Plant species produce a wide variety of antimicrobial metabolites to protect themselves against potential pathogens in natural environments. Phytoalexins are low molecular weight compounds produced by plants in response to attempted attacks of pathogens. Accumulation of phytoalexins in attacked plant tissues can inhibit the growth of penetrating pathogens. Thus phytoalexins play a major role in post-invasion defense against pathogens. Major phytoalexins produced by Solanaceous plants are sesquiterpenoids such as capsidiol produced by Nicotiana and Capsicum species, and rishitin produced by Solanum species, which are synthesized in the cytosol and secreted into the intercellular space of plant tissues. We previously reported that deficiency in capsidiol secretion causes enhanced susceptibility of Nicotiana benthamiana to potato late blight pathogen, Phytophthora infestans . Here, we describe a practical protocol to measure the secreted capsidiol in N. benthamiana. (Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.) |
| Databáze: | MEDLINE |
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