Extraction of RNA from Recalcitrant Tree Species Paulownia elongata .
| Autor: | Ramadoss N; Department of Biology, California State University, Northridge, California, USA., Basu C; Department of Biology, California State University, Northridge, California, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Bio-protocol [Bio Protoc] 2018 Jul 20; Vol. 8 (14), pp. e2925. Date of Electronic Publication: 2018 Jul 20 (Print Publication: 2018). |
| DOI: | 10.21769/BioProtoc.2925 |
| Abstrakt: | Isolation of pure RNA is the basic requisite for most molecular biology work. Plants contain polyphenols and polysaccharides, which can interfere with isolation of pure RNA from them. Especially hardwood tree species like Paulownia elongata have surplus amount of RNA-binding alkaloids, proteins and secondary metabolites that can further complicate the process of RNA extraction. Paulownia elongata is a fast-growing tree species which is known for its role in environmental adaptability and biofuel research. Here we describe an economical, efficient and time-saving method (2 h) to extract RNA from leaf tissues of the tree Paulownia elongata . Lack of DNA contamination and good RNA integrity were confirmed using RNA Gel electrophoresis. The purity of RNA was confirmed using Nanodrop spectrophotometer that revealed an A (Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.) |
| Databáze: | MEDLINE |
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