Antibody cooperative adsorption onto AuNPs and its exploitation to force natural killer cells to kill HIV-infected T cells.

Autor: Astorga-Gamaza A; Infectious Disease Department, Hospital Universitario Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Vitali M; Infectious Disease Department, Hospital Universitario Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Borrajo ML; Infectious Disease Department, Hospital Universitario Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Suárez-López R; Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain., Jaime C; Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain., Bastus N; Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193 Barcelona, Spain.; Institució Catalana de Recerca i Estudis Avançats (ICREA), 08010 Barcelona, Spain., Serra-Peinado C; Infectious Disease Department, Hospital Universitario Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Luque-Ballesteros L; Infectious Disease Department, Hospital Universitario Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Blanch-Lombarte O; IrsiCaixa AIDS Research Institute, Badalona, Spain.; Germans Trias i Pujol Research Institute (IGTP), Universitat Autònoma de Barcelona, Badalona, Spain., Prado JG; IrsiCaixa AIDS Research Institute, Badalona, Spain.; Germans Trias i Pujol Research Institute (IGTP), Universitat Autònoma de Barcelona, Badalona, Spain., Lorente J; Otorhinolaryngology Department, Vall d'Hebron University Hospital, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain., Pumarola F; Otorhinolaryngology Department, Vall d'Hebron University Hospital, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain., Pellicer M; Otorhinolaryngology Department, Vall d'Hebron University Hospital, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain., Falcó V; Infectious Disease Department, Hospital Universitario Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Genescà M; Infectious Disease Department, Hospital Universitario Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain., Puntes V; Infectious Disease Department, Hospital Universitario Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain.; Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193 Barcelona, Spain.; Institució Catalana de Recerca i Estudis Avançats (ICREA), 08010 Barcelona, Spain., Buzon MJ; Infectious Disease Department, Hospital Universitario Vall d'Hebron, Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain.
Jazyk: angličtina
Zdroj: Nano today [Nano Today] 2021 Feb; Vol. 36. Date of Electronic Publication: 2020 Dec 20.
DOI: 10.1016/j.nantod.2020.101056
Abstrakt: HIV represents a persistent infection which negatively alters the immune system. New tools to reinvigorate different immune cell populations to impact HIV are needed. Herein, a novel nanotool for the specific enhancement of the natural killer (NK) immune response towards HIV-infected T-cells has been developed. Bispecific Au nanoparticles (BiAb-AuNPs), dually conjugated with IgG anti-HIVgp120 and IgG anti-human CD16 antibodies, were generated by a new controlled, linker-free and cooperative conjugation method promoting the ordered distribution and segregation of antibodies in domains. The cooperatively-adsorbed antibodies fully retained the capabilities to recognize their cognate antigen and were able to significantly enhance cell-to-cell contact between HIV-expressing cells and NK cells. As a consequence, the BiAb-AuNPs triggered a potent cytotoxic response against HIV-infected cells in blood and human tonsil explants. Remarkably, the BiAb-AuNPs were able to significantly reduce latent HIV infection after viral reactivation in a primary cell model of HIV latency. This novel molecularly-targeted strategy using a bispecific nanotool to enhance the immune system represents a new approximation with potential applications beyond HIV.
Databáze: MEDLINE
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