Full nucleotide sequencing of ribosomal DNA internal transcribed spacer of Leishmania species causing cutaneous leishmaniasis in Brazil and its potential for species typing.

Autor: Espada CR; Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil., Ferreira BA; Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, Brazil., Ortiz PA; Departamento de Biologia, Facultad de Ciencias Básicas, Universidad del Tolima, Tolima, Colombia., Uliana SRB; Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil., Coelho AC; Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, Brazil. Electronic address: accoelho@unicamp.br.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2021 Nov; Vol. 223, pp. 106093. Date of Electronic Publication: 2021 Aug 11.
DOI: 10.1016/j.actatropica.2021.106093
Abstrakt: Species-specific diagnosis still represents a challenge in leishmaniasis management, particularly in regions with multiple endemic species. In Brazil, seven species have been recognized as etiological agents of cutaneous leishmaniasis. The disease comprises complex clinical presentation patterns, classified as localized, diffuse, disseminated and mucocutaneous leishmaniasis. In this study, we characterized the full nucleotide sequence of a region comprising the ribosomal DNA internal transcribed spacers 1 and 2 and 5.8 S gene of reference strains of Leishmania (Viannia) species reported as causative agents of human leishmaniasis in Brazil. The analysis of the nucleotide sequence of this region was able to discriminate species in the Leishmania (Viannia) subgenus and to determine intra- and interspecies phylogenetic relationships.
(Copyright © 2021. Published by Elsevier B.V.)
Databáze: MEDLINE
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