Pyramiding ascorbate-glutathione pathway in Lycopersicum esculentum confers tolerance to drought and salinity stress.

Autor: Raja V; Plant Molecular Biology Laboratory, Department of Botany, University of Kashmir, Srinagar, Kashmir, 190006, India., Wani UM; Plant Molecular Biology Laboratory, Department of Botany, University of Kashmir, Srinagar, Kashmir, 190006, India.; Department of Biotechnology, University of Kashmir, Srinagar, 190006, India., Wani ZA; Department of Biotechnology, University of Kashmir, Srinagar, 190006, India., Jan N; Plant Molecular Biology Laboratory, Department of Botany, University of Kashmir, Srinagar, Kashmir, 190006, India., Kottakota C; International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi, 1100067, India., Reddy MK; International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi, 1100067, India., Kaul T; International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi, 1100067, India., John R; Plant Molecular Biology Laboratory, Department of Botany, University of Kashmir, Srinagar, Kashmir, 190006, India. riffatminhaj@kashmiruniversity.ac.in.
Jazyk: angličtina
Zdroj: Plant cell reports [Plant Cell Rep] 2022 Mar; Vol. 41 (3), pp. 619-637. Date of Electronic Publication: 2021 Aug 12.
DOI: 10.1007/s00299-021-02764-8
Abstrakt: Key Message: Stacking Glutathione-Ascorbate pathway genes (PgSOD, PgAPX, PgGR, PgDHAR and PgMDHAR) under stress inducible promoter RD29A imparts significant tolerance to drought and salinity stress in Solanum lycopersicum. Although the exposure of plants to different environmental stresses results in overproduction of reactive oxygen species (ROS), many plants have developed some unique systems to alleviate the ROS production and mitigate its deleterious effect. One of the key pathways that gets activated in plants is ascorbate glutathione (AsA-GSH) pathway. To demonstrate the effect of this pathway in tomato, we developed the AsA-GSH overexpression lines by stacking the genes of the AsA-GSH pathway genes isolated from Pennisetum glaucoma (Pg) including PgSOD, PgAPX, PgGR, PgDHAR and PgMDHAR under stress inducible promoter RD29A. The overexpression lines have an improved germination and seedling growth with concomitant elevation in the survival rate. The exposure of transgenic seedlings to varying stress regiments exhibited escalation in the antioxidant enzyme activity and lesser membrane damage as reflected by decreased electrolytic leakage and little accumulation of malondialdehyde and H 2 O 2 . Furthermore, the transgenic lines accumulated high levels of osmoprotectants with increase in the relative water content. The increased photosynthetic activity and enhanced gaseous exchange parameters further confirmed the enhanced tolerance of AsA-GSH overexpression lines. We concluded that pyramiding of AsA-GSH pathway genes is an effective strategy for developing stress resistant crops.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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