Lipidomics dataset of Danio rerio optic nerve regeneration model.
| Autor: | Arcuri J; Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, 33136, USA.; Miami Integrative Metabolomics Research Center, Miami, FL, 33136, USA.; Molecular Cellular Pharmacology Graduate Program, University of Miami, Miami, FL, 33136, USA., Veldman MB; Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA., Bhattacharya SK; Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, 33136, USA.; Miami Integrative Metabolomics Research Center, Miami, FL, 33136, USA.; Molecular Cellular Pharmacology Graduate Program, University of Miami, Miami, FL, 33136, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Data in brief [Data Brief] 2021 Jul 14; Vol. 37, pp. 107260. Date of Electronic Publication: 2021 Jul 14 (Print Publication: 2021). |
| DOI: | 10.1016/j.dib.2021.107260 |
| Abstrakt: | The right optic nerve of adult, 6 month to 1 year old, female and male Danio rerio were crushed and collected three days after. Matching controls of uninjured left optic nerves were also collected. The tissue was dissected from euthanized fish and frozen on dry ice. Samples were pooled for each category (female crush, female control, male crush, male control) n = 24 to obtain sufficient tissue for analysis. The brain from one male fish was also collected for control/calibration. Lipid extraction was done with the Bligh and Dyer [1] method, followed by untargeted liquid chromatography-mass spectrometry (LC MS-MS) lipid profiling using a Q-Exactive Orbitrap instrument coupled with Vanquish Horizon Binary UHPLC LC-MS system. The lipids were identified and quantified with LipidSearch 4.2.21 and the statistical analysis was conducted through Metaboanalyst 5.0. This data is available at Metabolomics Workbench, Study ID ST001725. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships which have, or could be perceived to have, influenced the work reported in this article. (© 2021 The Author(s).) |
| Databáze: | MEDLINE |
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