Enrichment of inflammatory mediators in the synovial fluid is associated with slower progression of mild to moderate osteoarthritis in the porcine knee.

Autor:	Karamchedu NP; Department of Orthopaedics, Warren Alpert Medical School of Brown University/Rhode Island Hospital Providence, RI, USA., Fleming BC; Department of Orthopaedics, Warren Alpert Medical School of Brown University/Rhode Island Hospital Providence, RI, USA., Donnenfield JI; Division of Sports Medicine, Department of Orthopaedic Surgery, Boston Children's Hospital, Harvard Medical School Boston, MA, USA., Proffen BL; Division of Sports Medicine, Department of Orthopaedic Surgery, Boston Children's Hospital, Harvard Medical School Boston, MA, USA., Costa MQ; Department of Orthopaedics, Warren Alpert Medical School of Brown University/Rhode Island Hospital Providence, RI, USA., Molino J; Department of Orthopaedics, Warren Alpert Medical School of Brown University/Rhode Island Hospital Providence, RI, USA., Murray MM; Division of Sports Medicine, Department of Orthopaedic Surgery, Boston Children's Hospital, Harvard Medical School Boston, MA, USA.
Jazyk:	angličtina
Zdroj:	American journal of translational research [Am J Transl Res] 2021 Jul 15; Vol. 13 (7), pp. 7667-7676. Date of Electronic Publication: 2021 Jul 15 (Print Publication: 2021).
Abstrakt:	The roles that cytokines and matrix metalloproteinases play in the onset and progression of posttraumatic osteoarthritis (PTOA) remain a topic of debate. The study objective was to evaluate the concentrations of these inflammatory mediators during the development of mild to moderate PTOA in the porcine anterior cruciate ligament (ACL) surgical model. We hypothesized that there would be more animals with detectable mediators in the pigs that develop moderate PTOA (those receiving ACL reconstruction or untreated ACL transection) compared to those that develop mild PTOA (those receiving scaffold-enhanced ACL repair). 36 Yucatan minipigs underwent ACL transection and were randomized to: 1) no further treatment, 2) ACL reconstruction, or 3) scaffold-enhanced ACL repair. Synovial fluid samples were obtained pre-operatively, and at 1, 4, 12, 26 and 52 weeks post-operatively. The concentrations of inflammatory mediator in the synovial fluid samples were evaluated via multiplex assay. Macroscopic cartilage assessments were performed following euthanasia at 52 weeks. As found in prior studies, the repair group had significantly less cartilage damage than either the ACL transected or ACL reconstruction groups (P<.03). The presence and concentrations of the biomarkers were influenced by surgical group and time. In general, the concentrations of inflammatory mediators were higher in the repair group, which exhibited less cartilage damage than the other two treatment groups. While this finding disproved the hypotheses, these data suggest that the metabolic activity of the joints exhibiting less cartilage damage remained higher over the 52-week period than those that did not. Competing Interests: It should be noted that Dr. Murray is a founder, paid consultant and equity holder, Dr. Proffen is a paid consultant and equity holder, and Dr. Fleming is a founder of Miach Orthopaedics, Inc, which was formed to upscale production of a scaffold for ACL repair and is related to one of the ACL procedures described herein. Drs. Murray and Proffen maintain a conflict-of-interest management plan approved by Boston Children’s Hospital and Harvard Medical School. Dr. Fleming also maintains a conflict-of-interest management plan with Rhode Island Hospital with similar oversight. (AJTR Copyright © 2021.)
Databáze:	MEDLINE
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