| Autor: |
Tohma K; Division of Viral Products, CBER, FDA, Silver Spring, MD, USA., Saito M; Department of Virology, Tohoku University Graduate School of Medicine, Sendai, Japan., Pajuelo MJ; Department of Cellular and Molecular Sciences, Faculty of Sciences, Universidad Peruana Cayetano Heredia, Lima, Peru., Mayta H; Department of Cellular and Molecular Sciences, Faculty of Sciences, Universidad Peruana Cayetano Heredia, Lima, Peru., Zimic M; Department of Cellular and Molecular Sciences, Faculty of Sciences, Universidad Peruana Cayetano Heredia, Lima, Peru., Lepore CJ; Division of Viral Products, CBER, FDA, Silver Spring, MD, USA., Ford-Siltz LA; Division of Viral Products, CBER, FDA, Silver Spring, MD, USA., Gilman RH; Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA., Parra GI; Division of Viral Products, CBER, FDA, Silver Spring, MD, USA. |
| Abstrakt: |
Norovirus is a major cause of acute gastroenteritis. Human noroviruses present >30 different genotypes, with a single genotype (GII.4) predominating worldwide. Concurrent outbreaks of norovirus are often associated with the emergence of new viruses. While different hypotheses have been presented, the source of new mutations in noroviruses is still unknown. In this study, we applied high-resolution sequencing to determine the intra-host viral diversity presented by noroviruses during the acute and shedding phase of infection in children. Profiling viral intra-host diversification at nearly full genome level indicated that GII.4 viruses presented dynamic intra-host variation, while non-GII.4 viruses presented minimal variation throughout the infection. Notably, the intra-host genetic variation during the shedding phase recapitulates the genetic diversity observed at the global level, particularly those mapping at the VP1 antigenic sites. Thus the intra-host evolution in healthy children explains the source of norovirus mutations that results in diversification at the global scale. |
