CD19 + CD21 lo/neg cells are increased in systemic sclerosis-associated interstitial lung disease.

Autor: Wilfong EM; Division of Allergy, Pulmonary, and Critical Care Medicine, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.; Division of Rheumatology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA., Vowell KN; Division of Rheumatology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA., Bunn KE; Deparment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA., Rizzi E; Division of Allergy, Pulmonary, and Critical Care Medicine, Vanderbilt University Medical Center, Nashville, TN, 37232, USA., Annapureddy N; Division of Rheumatology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA., Dudenhofer RB; Division of Allergy, Pulmonary, and Critical Care Medicine, Vanderbilt University Medical Center, Nashville, TN, 37232, USA., Barnado A; Division of Rheumatology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA., Bonami RH; Division of Rheumatology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.; Deparment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.; Vanderbilt Institute for Infection, Immunology, and Inflammation, Nashville, TN, USA., Johnson JE; Deparment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA., Crofford LJ; Division of Rheumatology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.; Deparment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.; Vanderbilt Institute for Infection, Immunology, and Inflammation, Nashville, TN, USA., Kendall PL; Division of Allergy, Pulmonary, and Critical Care Medicine, Vanderbilt University Medical Center, Nashville, TN, 37232, USA. peggy.kendall@wustl.edu.; Deparment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA. peggy.kendall@wustl.edu.; Vanderbilt Institute for Infection, Immunology, and Inflammation, Nashville, TN, USA. peggy.kendall@wustl.edu.; Division of Allergy and Immunology, Department of Medicine, Washington University School of Medicine, 660 S. Euclid Ave, Campus Box 8122, St. Louis, MO, 63110, USA. peggy.kendall@wustl.edu.
Jazyk: angličtina
Zdroj: Clinical and experimental medicine [Clin Exp Med] 2022 May; Vol. 22 (2), pp. 209-220. Date of Electronic Publication: 2021 Aug 10.
DOI: 10.1007/s10238-021-00745-5
Abstrakt: Interstitial lung disease (ILD) represents a significant cause of morbidity and mortality in systemic sclerosis (SSc). The purpose of this study was to examine recirculating lymphocytes from SSc patients for potential biomarkers of interstitial lung disease (ILD). Peripheral blood mononuclear cells (PBMCs) were isolated from patients with SSc and healthy controls enrolled in the Vanderbilt University Myositis and Scleroderma Treatment Initiative Center cohort between 9/2017-6/2019. Clinical phenotyping was performed by chart abstraction. Immunophenotyping was performed using both mass cytometry and fluorescence cytometry combined with t-distributed stochastic neighbor embedding analysis and traditional biaxial gating. This study included 34 patients with SSc-ILD, 14 patients without SSc-ILD, and 25 healthy controls. CD21 lo/neg cells are significantly increased in SSc-ILD but not in SSc without ILD (15.4 ± 13.3% vs. 5.8 ± 0.9%, p = 0.002) or healthy controls (5.0 ± 0.5%, p < 0.0001). While CD21 lo/neg B cells can be identified from a single biaxial gate, tSNE analysis reveals that the biaxial gate is comprised of multiple distinct subsets, all of which are increased in SSc-ILD. CD21 lo/neg cells in both healthy controls and SSc-ILD are predominantly tBET positive and do not have intracellular CD21. Immunohistochemistry staining demonstrated that CD21 lo/neg B cells diffusely infiltrate the lung parenchyma of an SSc-ILD patient. Additional work is needed to validate this biomarker in larger cohorts and longitudinal studies and to understand the role of these cells in SSc-ILD.
(© 2021. The Author(s).)
Databáze: MEDLINE
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