Genetic surveillance in the Greater Mekong subregion and South Asia to support malaria control and elimination.

Autor:	Jacob CG; Wellcome Sanger Institute, Hinxton, United Kingdom., Thuy-Nhien N; Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam., Mayxay M; Lao-Oxford-Mahosot Hospital-Wellcome Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.; Institute of Research and Education Development (IRED), University of Health Sciences, Ministry of Health, Vientiane, Lao People's Democratic Republic.; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom., Maude RJ; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand.; Harvard TH Chan School of Public Health, Harvard University, Boston, United States., Quang HH; Institute of Malariology, Parasitology and Entomology (IMPE-QN), Quy Nhon, Viet Nam., Hongvanthong B; Centre of Malariology, Parasitology, and Entomology, Vientiane, Lao People's Democratic Republic., Vanisaveth V; Centre of Malariology, Parasitology, and Entomology, Vientiane, Lao People's Democratic Republic., Ngo Duc T; National Institute of Malariology, Parasitology and Entomology (NIMPE), Hanoi, Viet Nam., Rekol H; National Center for Parasitology, Entomology, and Malaria Control, Phnom Penh, Cambodia., van der Pluijm R; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand., von Seidlein L; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand., Fairhurst R; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, United States., Nosten F; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Shoklo Malaria Research Unit, Mae Sot, Thailand., Hossain MA; Chittagong Medical College Hospital, Chittagong, Bangladesh., Park N; Wellcome Sanger Institute, Hinxton, United Kingdom., Goodwin S; Wellcome Sanger Institute, Hinxton, United Kingdom., Ringwald P; World Health Organization, Geneva, Switzerland., Chindavongsa K; Centre of Malariology, Parasitology, and Entomology, Vientiane, Lao People's Democratic Republic., Newton P; Lao-Oxford-Mahosot Hospital-Wellcome Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand., Ashley E; Lao-Oxford-Mahosot Hospital-Wellcome Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom., Phalivong S; Lao-Oxford-Mahosot Hospital-Wellcome Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic., Maude R; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand.; Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand., Leang R; National Center for Parasitology, Entomology, and Malaria Control, Phnom Penh, Cambodia., Huch C; National Center for Parasitology, Entomology, and Malaria Control, Phnom Penh, Cambodia., Dong LT; Institute of Malariology, Parasitology and Entomology (IMPEHCM), Ho Chi Minh City, Viet Nam., Nguyen KT; Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam., Nhat TM; Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam., Hien TT; Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam., Nguyen H; Vysnova Partners Inc, Hanoi, Viet Nam., Zdrojewski N; Vysnova Partners Inc, Hanoi, Viet Nam., Canavati S; Vysnova Partners Inc, Hanoi, Viet Nam., Sayeed AA; Chittagong Medical College Hospital, Chittagong, Bangladesh., Uddin D; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand., Buckee C; Harvard TH Chan School of Public Health, Harvard University, Boston, United States., Fanello CI; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand., Onyamboko M; Kinshasa School of Public Health, University of Kinshasa, Kinshasa, Democratic Republic of the Congo., Peto T; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand., Tripura R; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand., Amaratunga C; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, United States., Myint Thu A; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Shoklo Malaria Research Unit, Mae Sot, Thailand., Delmas G; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Shoklo Malaria Research Unit, Mae Sot, Thailand., Landier J; Shoklo Malaria Research Unit, Mae Sot, Thailand.; Aix-Marseille Université, INSERM, IRD, SESSTIM, Aix Marseille Institute of Public Health, ISSPAM, Marseille, France., Parker DM; Shoklo Malaria Research Unit, Mae Sot, Thailand.; Susan and Henry Samueli College of Health Sciences, University of California, Irvine, Irvine, United States., Chau NH; Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam., Lek D; National Center for Parasitology, Entomology, and Malaria Control, Phnom Penh, Cambodia., Suon S; National Center for Parasitology, Entomology, and Malaria Control, Phnom Penh, Cambodia., Callery J; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand., Jittamala P; Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand., Hanboonkunupakarn B; Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand., Pukrittayakamee S; Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.; The Royal Society of Thailand, Bangkok, Thailand., Phyo AP; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Myanmar-Oxford Clinical Research Unit, Yangon, Myanmar., Smithuis F; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Myanmar-Oxford Clinical Research Unit, Yangon, Myanmar., Lin K; Department of Medical Research, Pyin Oo Lwin, Myanmar., Thant M; Defence Services Medical Research Centre, Yangon, Myanmar., Hlaing TM; Defence Services Medical Research Centre, Yangon, Myanmar., Satpathi P; Midnapore Medical College, Midnapur, India., Satpathi S; Ispat General Hospital, Rourkela, India., Behera PK; Ispat General Hospital, Rourkela, India., Tripura A; Agartala Medical College, Agartala, India., Baidya S; Agartala Medical College, Agartala, India., Valecha N; National Institute of Malaria Research, Indian Council of Medical Research, New Delhi, India., Anvikar AR; National Institute of Malaria Research, Indian Council of Medical Research, New Delhi, India., Ul Islam A; Ramu Upazila Health Complex, Cox's Bazar, Bangladesh., Faiz A; Malaria Research Group and Dev Care Foundation, Dhaka, Bangladesh., Kunasol C; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand., Drury E; Wellcome Sanger Institute, Hinxton, United Kingdom., Kekre M; Wellcome Sanger Institute, Hinxton, United Kingdom., Ali M; Wellcome Sanger Institute, Hinxton, United Kingdom., Love K; Wellcome Sanger Institute, Hinxton, United Kingdom., Rajatileka S; Wellcome Sanger Institute, Hinxton, United Kingdom., Jeffreys AE; Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom., Rowlands K; Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom., Hubbart CS; Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom., Dhorda M; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand.; Worldwide Antimalarial Resistance Network (WWARN), Asia Regional Centre, Bangkok, Thailand., Vongpromek R; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand.; Worldwide Antimalarial Resistance Network (WWARN), Asia Regional Centre, Bangkok, Thailand., Kotanan N; Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand., Wongnak P; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand., Almagro Garcia J; MRC Centre for Genomics and Global Health, Big Data Institute, Oxford University, Oxford, United Kingdom., Pearson RD; Wellcome Sanger Institute, Hinxton, United Kingdom.; MRC Centre for Genomics and Global Health, Big Data Institute, Oxford University, Oxford, United Kingdom., Ariani CV; Wellcome Sanger Institute, Hinxton, United Kingdom., Chookajorn T; Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand., Malangone C; Wellcome Sanger Institute, Hinxton, United Kingdom., Nguyen T; Wellcome Sanger Institute, Hinxton, United Kingdom., Stalker J; Wellcome Sanger Institute, Hinxton, United Kingdom., Jeffery B; MRC Centre for Genomics and Global Health, Big Data Institute, Oxford University, Oxford, United Kingdom., Keatley J; Wellcome Sanger Institute, Hinxton, United Kingdom., Johnson KJ; Wellcome Sanger Institute, Hinxton, United Kingdom.; MRC Centre for Genomics and Global Health, Big Data Institute, Oxford University, Oxford, United Kingdom., Muddyman D; Wellcome Sanger Institute, Hinxton, United Kingdom., Chan XHS; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand., Sillitoe J; Wellcome Sanger Institute, Hinxton, United Kingdom., Amato R; Wellcome Sanger Institute, Hinxton, United Kingdom., Simpson V; Wellcome Sanger Institute, Hinxton, United Kingdom.; MRC Centre for Genomics and Global Health, Big Data Institute, Oxford University, Oxford, United Kingdom., Gonçalves S; Wellcome Sanger Institute, Hinxton, United Kingdom., Rockett K; Wellcome Sanger Institute, Hinxton, United Kingdom.; Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom., Day NP; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand., Dondorp AM; Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand., Kwiatkowski DP; Wellcome Sanger Institute, Hinxton, United Kingdom.; MRC Centre for Genomics and Global Health, Big Data Institute, Oxford University, Oxford, United Kingdom., Miotto O; Wellcome Sanger Institute, Hinxton, United Kingdom.; Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, Thailand.; MRC Centre for Genomics and Global Health, Big Data Institute, Oxford University, Oxford, United Kingdom.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 Aug 10; Vol. 10. Date of Electronic Publication: 2021 Aug 10.
DOI:	10.7554/eLife.62997
Abstrakt:	Background: National Malaria Control Programmes (NMCPs) currently make limited use of parasite genetic data. We have developed GenRe-Mekong, a platform for genetic surveillance of malaria in the Greater Mekong Subregion (GMS) that enables NMCPs to implement large-scale surveillance projects by integrating simple sample collection procedures in routine public health procedures. Methods: Samples from symptomatic patients are processed by SpotMalaria, a high-throughput system that produces a comprehensive set of genotypes comprising several drug resistance markers, species markers and a genomic barcode. GenRe-Mekong delivers Genetic Report Cards, a compendium of genotypes and phenotype predictions used to map prevalence of resistance to multiple drugs. Results: GenRe-Mekong has worked with NMCPs and research projects in eight countries, processing 9623 samples from clinical cases. Monitoring resistance markers has been valuable for tracking the rapid spread of parasites resistant to the dihydroartemisinin-piperaquine combination therapy. In Vietnam and Laos, GenRe-Mekong data have provided novel knowledge about the spread of these resistant strains into previously unaffected provinces, informing decision-making by NMCPs. Conclusions: GenRe-Mekong provides detailed knowledge about drug resistance at a local level, and facilitates data sharing at a regional level, enabling cross-border resistance monitoring and providing the public health community with valuable insights. The project provides a rich open data resource to benefit the entire malaria community. Funding: The GenRe-Mekong project is funded by the Bill and Melinda Gates Foundation (OPP11188166, OPP1204268). Genotyping and sequencing were funded by the Wellcome Trust (098051, 206194, 203141, 090770, 204911, 106698/B/14/Z) and Medical Research Council (G0600718). A proportion of samples were collected with the support of the UK Department for International Development (201900, M006212), and Intramural Research Program of the National Institute of Allergy and Infectious Diseases. Competing Interests: CJ, NT, MM, RM, HQ, BH, VV, TN, HR, Rv, Lv, RF, FN, MH, NP, SG, PR, KC, PN, EA, SP, RM, RL, CH, LD, KN, TN, TH, AS, DU, CB, CF, MO, TP, RT, CA, AM, GD, JL, DP, NC, DL, SS, JC, PJ, BH, SP, AP, FS, KL, MT, TH, PS, SS, PB, AT, SB, NV, AA, AU, AF, CK, ED, MK, MA, KL, SR, AJ, KR, CH, MD, RV, NK, PW, JA, RP, CA, TC, CM, TN, JS, BJ, JK, KJ, DM, XC, JS, RA, VS, SG, KR, ND, AD, DK, OM No competing interests declared, HN, NZ, SC is an employee of Vysnova Partners Inc
Databáze:	MEDLINE
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