Evaluation of ELISA-Based Multiplex Peptides for the Detection of Human Serum Antibodies Induced by Zika Virus Infection across Various Countries.

Autor: Martinez Viedma MDP; J. Craig Venter Institute, La Jolla, CA 92137, USA., Panossian S; J. Craig Venter Institute, Rockville, MD 20850, USA., Gifford K; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA., García K; Instituto de Investigacion en Microbiologia, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras., Figueroa I; Instituto de Investigacion en Microbiologia, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras., Parham L; Instituto de Investigacion en Microbiologia, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras., de Moraes L; Institute Goncalo Moniz, Fiocruz Bahia, Salvador 40296-710, Brazil., Nunes Gomes L; Institute Goncalo Moniz, Fiocruz Bahia, Salvador 40296-710, Brazil., García-Salum T; Departmento de Enfermedades Infecciosas e Inmunología Pediátrica, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago H955+8Q, Chile., Perret C; Departmento de Enfermedades Infecciosas e Inmunología Pediátrica, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago H955+8Q, Chile., Weiskopf D; Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, La Jolla, CA 92037, USA., Tan GS; J. Craig Venter Institute, La Jolla, CA 92137, USA.; Department of Medicine, Infectious Diseases Division, University of California San Diego, La Jolla, CA 92037, USA., Augusto Silva A; Hospital Universitário-Universidade Federal do Maranhão, São Luís 65000-000, Brazil., Boaventura V; Institute Goncalo Moniz, Fiocruz Bahia, Salvador 40296-710, Brazil.; Faculdade de Medicina da Bahia-Universidade Federal da Bahia, Salvador 40000-000, Brazil., Ruiz-Palacios GM; Instituto Nacional de Nutricion, Universidad Nacional Autonoma de Mexico, Mexico City 04510, Mexico., Sette A; Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, La Jolla, CA 92037, USA.; Department of Medicine, University of California San Diego, La Jolla, CA 92037, USA., De Silva AD; Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, La Jolla, CA 92037, USA.; Genetech Research Institute, Colombo 00800, Sri Lanka.; Department of Paraclinical Sciences, Faculty of Medicine, General Sir John Kotelawala Defence University, Ratmalana 10390, Sri Lanka., Medina RA; Departmento de Enfermedades Infecciosas e Inmunología Pediátrica, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago H955+8Q, Chile.; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA., Lorenzana I; Instituto de Investigacion en Microbiologia, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras., Akrami KM; Faculdade de Medicina da Bahia-Universidade Federal da Bahia, Salvador 40000-000, Brazil.; Department of Medicine, University of California San Diego, La Jolla, CA 92037, USA., Khouri R; Faculdade de Medicina da Bahia-Universidade Federal da Bahia, Salvador 40000-000, Brazil., Olson D; Department of Pediatrics, Section of Infectious Diseases, University of Colorado School of Medicine, Aurora, CO 80045, USA., Pickett BE; J. Craig Venter Institute, La Jolla, CA 92137, USA.; J. Craig Venter Institute, Rockville, MD 20850, USA.; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2021 Jul 08; Vol. 13 (7). Date of Electronic Publication: 2021 Jul 08.
DOI: 10.3390/v13071319
Abstrakt: Zika virus (ZIKV) is a mosquito-borne Flavivirus with a positive-sense RNA genome, which are generally transmitted through the bite of an infected Aedes mosquito. ZIKV infections could be associated with neurological sequelae that, and otherwise produces similar clinical symptoms as other co-circulating pathogens. Past infection with one member of the Flavivirus genus often induces cross-reactive antibodies against other flaviruses. These attributes complicate the ability to differentially diagnose ZIKV infection from other endemic mosquito-borne viruses, making it both a public health issue as well as a diagnostic challenge. We report the results from serological analyses using arbovirus-specific peptides on 339 samples that were previously collected from 6 countries. Overall, we found that our multiplexed peptide-based ELISA was highly efficient for identifying ZIKV antibodies as early as 2 weeks post infection, and that it correlates with microneutralization, plaque reduction neutralization tests (PRNTs) and commercial tests for ZIKV in previously characterized samples. We observed that seropositivity varied by patient cohort, reflecting the sampling period in relation to the 2015-2016 ZIKV outbreak. This work evaluates the accuracy, specificity, and sensitivity of our peptide-based ELISA method for detecting ZIKV antibodies from geographically diverse regions. These findings can contribute to ongoing serological methods development and can be adapted for use in future studies.
Databáze: MEDLINE
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