| Autor: |
Beliaev DV; K. A. Timiryazev Institute of Plant Physiology RAS, 127276 Moscow, Russia., Yuorieva NO; K. A. Timiryazev Institute of Plant Physiology RAS, 127276 Moscow, Russia., Tereshonok DV; K. A. Timiryazev Institute of Plant Physiology RAS, 127276 Moscow, Russia., Tashlieva II; K. A. Timiryazev Institute of Plant Physiology RAS, 127276 Moscow, Russia., Derevyagina MK; Russian Potato Research Center, 140052 Kraskovo, Russia., Meleshin AA; Russian Potato Research Center, 140052 Kraskovo, Russia., Rogozhin EA; M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, 117997 Moscow, Russia.; All-Russian Institute of Plant Protection, 196608 St.-Petersburg-Pushkin, Russia., Kozlov SA; M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, 117997 Moscow, Russia. |
| Abstrakt: |
In the common chickweed Stellaria media , two antimicrobial peptides (AMPs), SmAMP1.1a and SmAMP1.2a, have been shown to be proteolytically released as products of the expression of a single gene, proSmAMP1 . In this study, the gene proSmAMP1 was introduced into two potato varieties, Zhukovsky ranny and Udacha. These early-maturing varieties were shown to be susceptible to early blight caused by Alternaria spp. Most transgenic lines of either variety having strong expression of the target gene demonstrated high levels of resistance to Alternaria spp. during three years of cultivation, but did not otherwise differ from the initial varieties. Disease severity index (DSI) was introduced as a complex measure of plant susceptibility to early blight, taking into account the diameter of lesions caused by the Alternaria spp., the fungus sporulation intensity and its incubation period duration. Across all transgenic lines, the DSI inversely correlated both with the target gene expression and the copy number in the plant genome. Our results are promising for improving the resistance of potato and other crops to early blight by expression of AMPs from wild plants. |
