Cardioporation enhances myocardial gene expression in rat heart.

Autor: Boye C; Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, United States., Arpag S; LifeNet Health, Virginia Beach, VA, United States., Burcus N; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, United States., Lundberg C; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, United States., DeClemente S; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, United States., Heller R; Department of Medical Engineering, University of South Florida, Tampa, FL, United States., Francis M; Asante Bio, Norfolk, VA, United States., Bulysheva A; Department of Electrical and Computer Engineering, Old Dominion University, United States.
Jazyk: angličtina
Zdroj: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2021 Dec; Vol. 142, pp. 107892. Date of Electronic Publication: 2021 Jul 27.
DOI: 10.1016/j.bioelechem.2021.107892
Abstrakt: Damage from myocardial infarction (MI) and subsequent heart failure are serious public health concerns. Current clinical treatments and therapies to treat MI damage largely do not address the regeneration of cardiomyocytes. In a previous study, we established that it is possible to promote regeneration of cardiac muscle with vascular endothelial growth factor B gene delivery directly to the ischemic myocardium. In the current study we aim to optimize cardioporation parameters to increase expression efficiency by varying electrode configuration, applied voltage, pulse length, and plasmid vector size. By using a surface monopolar electrode, optimized pulsing conditions and reducing vector size, we were able to prevent ventricular fibrillation, increase survival, reduce tissue damage, and significantly increase gene expression levels.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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