Circulating microRNA breast cancer biomarker detection in patient sera with surface plasmon resonance imaging biosensor.

Autor: Wong CL; Translational Biophotonic Laboratory, Institute of Bioengineering and Bioimaging, Agency of Science, Technology and Research (A*STAR), Singapore., Loke SY; Division of Cellular and Molecular Research, Humphrey Oei Institute of Cancer Research, National Cancer Centre Singapore (NCCS), Singapore., Lim HQ; Translational Biophotonic Laboratory, Institute of Bioengineering and Bioimaging, Agency of Science, Technology and Research (A*STAR), Singapore., Balasundaram G; Translational Biophotonic Laboratory, Institute of Bioengineering and Bioimaging, Agency of Science, Technology and Research (A*STAR), Singapore., Chan P; Division of Cellular and Molecular Research, Humphrey Oei Institute of Cancer Research, National Cancer Centre Singapore (NCCS), Singapore., Chong BK; Department of General Surgery, Tan Tock Seng Hospital, Singapore., Tan EY; Department of Diagnostic Radiology, Tan Tock Seng Hospital, Singapore.; Lee Kong Chian School of Medicine, Singapore.; Institute of Molecular and Cell Biology, Agency of Science, Technology and Research (A*STAR), Singapore., Lee ASG; Division of Cellular and Molecular Research, Humphrey Oei Institute of Cancer Research, National Cancer Centre Singapore (NCCS), Singapore., Olivo M; Translational Biophotonic Laboratory, Institute of Bioengineering and Bioimaging, Agency of Science, Technology and Research (A*STAR), Singapore.
Jazyk: angličtina
Zdroj: Journal of biophotonics [J Biophotonics] 2021 Nov; Vol. 14 (11), pp. e202100153. Date of Electronic Publication: 2021 Aug 23.
DOI: 10.1002/jbio.202100153
Abstrakt: In this article, we report for the first time, the detection of circulating miRNA as a breast cancer biomarker in patient sera using surface plasmon resonance imaging biosensor. The advantage of this approach lies in the rapid, label-free and sensitive detection. The sensor excites plasmonic resonance on the gold sensor surface and specific DNA-miRNA molecular bindings elucidate responses in the plasmonic resonance image. Experiments of detecting synthetic miRNA molecules (miR-1249) were performed and the sensor resolution was found to be 63.5 nM. The sensor was further applied to screen 17 patient serum samples from National Cancer Centre Singapore and Tan Tock Seng Hospital. Sensor intensity response was found to differ by 20% between malignant and benign cases and thus forms, a potential and an important metric in distinguishing benignity and malignancy.
(© 2021 Wiley-VCH GmbH.)
Databáze: MEDLINE
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