Evaluation of DNA extraction methods for molecular traceability in cold pressed, solvent extracted and refined groundnut oils.

Autor: Bojang KP; Post Graduate & Research Centre, PJTS Agricultural University, Hyderabad, India., Kuna A; 2MFPI-Quality Control Laboratory, PJTS Agricultural University, Hyderabad, India., Pushpavalli SNCVL; Institute of Biotechnology, PJTS Agricultural University, Hyderabad, India., Sarkar S; Departmentof Foods & Nutrition, College of Community Science, PJTS Agricultural University, Hyderabad, India., Sreedhar M; 5Regional Rice and Sugarcane Research Centre, PJTS Agricultural University, Rudrur, India.
Jazyk: angličtina
Zdroj: Journal of food science and technology [J Food Sci Technol] 2021 Sep; Vol. 58 (9), pp. 3561-3567. Date of Electronic Publication: 2021 Mar 24.
DOI: 10.1007/s13197-021-05079-4
Abstrakt: Groundnut oil (GNO)/peanut oil is one of the agro-food products with great economic value and hence an attractive target for adulteration and mislabeling. Simple Sequence Repeats (SSR) are markers of choice for DNA fingerprinting studies as they exhibit high polymorphism due to variable number of repeats. Hence, this study was designed to evaluate and optimize a method for DNA isolation from groundnut oil and study the possibility of using the isolated DNA for molecular traceability using SSR markers. Four methods to isolate DNA from groundnut oil were evaluated. All the four methods were modified CTAB protocols, but differed in procedures for extraction, buffer compositions, amount of oil used and DNA carriers. For molecular traceability of oils, extraction and recovery of DNA from edible oil is a key step, especially in refined oils. A method that employed DNA enrichment prior to extraction with CTAB buffer yielded amplifiable DNA from cold pressed GNO, crude hexane extracted GNO and refined GNO. The optimized method for isolation of DNA from groundnut oil is simple, efficient, less costly and reproducible when compared to chromatography and spectroscopy based techniques.
Competing Interests: Conflict of interestThe authors report no conflicts of interest. The authors alone are responsible for the content, writing of the paper and complete responsibility for the integrity and accuracy of the data presented.
(© Association of Food Scientists & Technologists (India) 2021.)
Databáze: MEDLINE
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