Evaluation of the genotoxic, mutagenic, and histopathological hepatic effects of polyoxyethylene amine (POEA) and glyphosate on Dendropsophus minutus tadpoles.

Autor: Lopes A; Laboratory of Mutagenesis, Institute of Biological Sciences, ICB I - Federal University of Goiás, Samambaia Campus, Goiânia, Goiás, CEP: 74690-900, Brazil. Electronic address: alice_tcl@discente.ufg.br., Benvindo-Souza M; Laboratory of Mutagenesis, Institute of Biological Sciences, ICB I - Federal University of Goiás, Samambaia Campus, Goiânia, Goiás, CEP: 74690-900, Brazil. Electronic address: marcelinobenvindo@discente.ufg.br., Carvalho WF; Laboratory of Mutagenesis, Institute of Biological Sciences, ICB I - Federal University of Goiás, Samambaia Campus, Goiânia, Goiás, CEP: 74690-900, Brazil. Electronic address: wanessa.biologia@hotmail.com., Nunes HF; Laboratory of Mutagenesis, Institute of Biological Sciences, ICB I - Federal University of Goiás, Samambaia Campus, Goiânia, Goiás, CEP: 74690-900, Brazil. Electronic address: hugofreire@ufg.br., de Lima PN; Laboratory of Human and Animal Morphology, Institute of Biological Sciences, ICB III - Federal University of Goiás, Samambaia Campus, Goiânia, Goiás, CEP: 74690-900, Brazil. Electronic address: phamellaneres@discente.ufg.br., Costa MS; Laboratory of Human and Animal Morphology, Institute of Biological Sciences, ICB III - Federal University of Goiás, Samambaia Campus, Goiânia, Goiás, CEP: 74690-900, Brazil. Electronic address: matheus_santos@discente.ufg.br., Benetti EJ; Laboratory of Human and Animal Morphology, Institute of Biological Sciences, ICB III - Federal University of Goiás, Samambaia Campus, Goiânia, Goiás, CEP: 74690-900, Brazil. Electronic address: ebenetti@ufg.br., Guerra V; Graduate Program in Ecology and Natural Resource Management, Center for Biological and Natural Sciences, Federal University of Acre, Rio Branco Campus, Rio Branco, Acre, CEP: 69915-900, Brazil. Electronic address: vinicius.batista@ufac.br., Saboia-Morais SMT; Cell Behavior Laboratory, Institute of Biological Sciences IV - Federal University of Goiás, Samambaia Campus, Goiânia, Goiás, CEP: 74690-900, Brazil. Electronic address: simone_saboia@ufg.br., Santos CE; Laboratory of Herpetology and Animal Behavior, Institute of Biological Sciences, ICB V - Federal University of Goiás, Samambaia Campus, Goiânia, Goiás, CEP: 74690-900, Brazil. Electronic address: carolinaemilia@discente.ufg.br., Simões K; Laboratory of Human and Animal Morphology, Institute of Biological Sciences, ICB III - Federal University of Goiás, Samambaia Campus, Goiânia, Goiás, CEP: 74690-900, Brazil. Electronic address: simoesk@ufg.br., Bastos RP; Laboratory of Herpetology and Animal Behavior, Institute of Biological Sciences, ICB V - Federal University of Goiás, Samambaia Campus, Goiânia, Goiás, CEP: 74690-900, Brazil. Electronic address: sapo06@ufg.br., de Melo E Silva D; Laboratory of Mutagenesis, Institute of Biological Sciences, ICB I - Federal University of Goiás, Samambaia Campus, Goiânia, Goiás, CEP: 74690-900, Brazil. Electronic address: danielamelosilva@ufg.br.
Jazyk: angličtina
Zdroj: Environmental pollution (Barking, Essex : 1987) [Environ Pollut] 2021 Nov 15; Vol. 289, pp. 117911. Date of Electronic Publication: 2021 Aug 04.
DOI: 10.1016/j.envpol.2021.117911
Abstrakt: Herbicides improve the productivity of a monoculture by eliminating weeds, although they may also be toxic and have negative effects on non-target organisms, such as amphibians. The present study evaluated the genotoxic, mutagenic, and histopathological hepatic responses of Dendropsophus minutus tadpoles to acute exposure (96 h) to the herbicide glyphosate (GLY, 65, 130, 260 and 520 μg/L) and the surfactant polyoxyethylene amine (POEA, 1.25, 2.5, 5 and 10 μg/L). On average, 174 % more genomic damage was observed in the tadpoles exposed to all concentrations of POEA in comparison with the control, while up to seven times more micronuclei were recorded, on average, at a concentration of 5 μg/L of POEA. All the individuals exposed to 10 μg/L of POEA died. The tadpoles exposed to GLY presented 165 % more DNA damage than the control, on average, at the highest concentrations (260 and 520 μg/L), and up to six times more micronuclei at 520 μg/L. The Erythrocyte Nuclear Abnormality test (ENA) detected a relatively high frequency of cells with lobed nuclei in the tadpoles expose to POEA at 5 μg/L and binucleated cells in those exposed to GLY at 520 μg/L. The hepatic histopathological observations revealed several types of lesions in the tadpoles exposed to both GLY and POEA. Overall, then, the results of the study indicate that both GLY and POEA have potential genotoxic, mutagenic, and hepatotoxic effects in D. minutus tadpoles. We emphasize the need for further studies to monitor the amphibian populations, such as those of D. minutus, which breed in aquatic environments associated with agricultural areas. The release of pollutants into natural habitats may have significant long-term impacts on the survival of anuran tadpoles.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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