Pegbelfermin, a PEGylated FGF21 analogue, has pharmacology without bone toxicity after 1-year dosing in skeletally-mature monkeys.

Autor: Thompson KE; Nonclinical Safety, Bristol-Myers Squibb, New Brunswick, NJ, USA., Guillot M; Musculoskeletal Research & Imaging, Charles River Laboratories, Senneville, QC, Canada., Graziano MJ; Nonclinical Safety, Bristol-Myers Squibb, New Brunswick, NJ, USA., Mangipudy RS; Nonclinical Safety, Bristol-Myers Squibb, New Brunswick, NJ, USA., Chadwick KD; Nonclinical Safety, Bristol-Myers Squibb, New Brunswick, NJ, USA. Electronic address: kristina.chadwick@bms.com.
Jazyk: angličtina
Zdroj: Toxicology and applied pharmacology [Toxicol Appl Pharmacol] 2021 Oct 01; Vol. 428, pp. 115673. Date of Electronic Publication: 2021 Aug 06.
DOI: 10.1016/j.taap.2021.115673
Abstrakt: Pegbelfermin (PGBF) is a PEGylated fibroblast growth factor 21 (FGF21) analogue in development for treatment of nonalcoholic steatohepatitis (NASH). Mouse models highlight potential utility of FGF21 in NASH, but also suggest negative effects on bone, though these findings are confounded by profound FGF21-related decreases in body mass/growth. This study aimed to profile PGBF-related bone effects in adult nonhuman primates after long-term, clinically-relevant exposures. Adult male cynomolgus monkeys received weekly subcutaneous PGBF (0.3, 0.75 mg/kg) or control injections for 1 year (n = 5/group). Assessments included body weight, clinical chemistry, adiponectin levels, bone turnover biomarkers, skeletal radiography, pharmacokinetics, immunogenicity, and histopathology. Bone densitometry and body composition were evaluated in vivo and/or ex vivo with dual-energy x-ray absorptiometry, peripheral quantitative computed tomography, and biomechanical strength testing. After 1 year of PGBF administration, there was clear evidence of sustained PGBF pharmacology in monkeys (peak increase in serum adiponectin of 1.7× and 2.35× pretest at 0.3 and 0.75 mg/kg PGBF, respectively) and decreased body weight compared with control at exposures comparable to those tested in humans. At 0.75 mg/kg PGBF, pharmacologically-mediated reductions in lean mass, lean area, and fat area were observed relative to controls. There were no PGBF-related effects on bone biomarkers, radiography, densitometry, or strength. Together, these data demonstrate that PGBF did not adversely alter bone metabolism, density, or strength following 1 year of dosing at clinically relevant (0.7-2.2× human AUC[0-168 h] at 20 mg once weekly), pharmacologically-active exposures in adult monkeys, suggesting a low potential for negative effects on bone quality in adult humans.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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