[Second-generation Sequencing Analysis of Ph + and Ph-like Childhood T-cell Acute Lymphoblastic Leukemia].

Autor:	Liao X; Department of Hematology and Oncology, Children's Hospital of Chongqing Medical University; Chongqing 400014, China,National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China., Zou PL; Department of Hematology and Oncology, Children's Hospital of Chongqing Medical University; Chongqing 400014, China,National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China., Shen YL; Department of Hematology and Oncology, Children's Hospital of Chongqing Medical University; Chongqing 400014, China,National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China., Guo YX; Department of Hematology and Oncology, Children's Hospital of Chongqing Medical University; Chongqing 400014, China,National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China., Song L; National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China,Department of Pharmacy, Children's Hospital of Chongqing Medical University, Chongqing 400014, China., Xia JW; Department of Hematology and Oncology, Children's Hospital of Chongqing Medical University; Chongqing 400014, China,E-mail: tomahawk6502@sohu.com.
Jazyk:	čínština
Zdroj:	Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2021 Aug; Vol. 29 (4), pp. 1101-1108.
DOI:	10.19746/j.cnki.issn.1009-2137.2021.04.013
Abstrakt:	Objective: To screen the core genes of Philadelphia chromosome positive/Ph like T-cell acute lymphoblastic leukemia (Ph + /Ph like T-ALL) using bioinformatics methods, and analyze the core sub-networks for exploration of the development process of Ph + /Ph like T-ALL, so as to find the molecular targets that may be used in clinical diagnosis and treatment. Methods: The WES/RNA-seq examination results of Ph + /Ph-like T-ALL children had be collected in our hospital, the genetic data that met the requirements had be downloaded from GEO database, then GRO2R online differentially expressed gene screening program was used to screen differentially expressed genes, finally, GO function enrichment analysis and KEGG pathway enrichment analysis were performed to compare differentially expressed genes. At the same time, STRING database and Cytoscape software were used to build protein interaction network and screen hub genes and core sub-networks. Results: For Ph + /Ph like T-ALL, a total of 84 differentially expressed genes had been found, for Ph + ALL a total of 249 differentially expressed genes, and for T-ALL a total of 175 differentially expressed genes. Based on the results of GO function enrichment, KEGG pathway enrichment analysis and protein interaction network, RPA1, POLD1, POLE and SOCS1 were selected as hub genes. DNA damage repair and JAK/STAT signal transduction pathway were the main functional sub-networks. Conclusion: There are obviously abnormal DNA damage repair pathways in children with Ph + /Ph like T-ALL. RPA1, POLD1 and POLE may be important relevant biomarkers for the occurrence and development of Ph + /Ph like T-ALL, which can provide a basis for further research.
Databáze:	MEDLINE
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