Placental 13 C-DHA metabolism and relationship with maternal BMI, glycemia and birthweight.

Autor: Watkins OC; Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, 1E Kent Ridge Road, NUHS Tower Block, Level 12, Singapore, 119228, Singapore., Selvam P; Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, 1E Kent Ridge Road, NUHS Tower Block, Level 12, Singapore, 119228, Singapore., Appukuttan Pillai R; Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, 1E Kent Ridge Road, NUHS Tower Block, Level 12, Singapore, 119228, Singapore., Cracknell-Hazra VKB; Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, 1E Kent Ridge Road, NUHS Tower Block, Level 12, Singapore, 119228, Singapore.; Singapore Institute for Clinical Sciences, Agency for Science, Technology and Research, Singapore, Singapore.; MRC Lifecourse Epidemiology Unit and NIHR Southampton Biomedical Research Centre, University of Southampton and University Hospital Southampton NHS Foundation Trust, Southampton, UK., Yong HEJ; Singapore Institute for Clinical Sciences, Agency for Science, Technology and Research, Singapore, Singapore., Sharma N; Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, 1E Kent Ridge Road, NUHS Tower Block, Level 12, Singapore, 119228, Singapore., Cazenave-Gassiot A; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.; Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, Singapore., Bendt AK; Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, Singapore., Godfrey KM; MRC Lifecourse Epidemiology Unit and NIHR Southampton Biomedical Research Centre, University of Southampton and University Hospital Southampton NHS Foundation Trust, Southampton, UK., Lewis RM; MRC Lifecourse Epidemiology Unit and NIHR Southampton Biomedical Research Centre, University of Southampton and University Hospital Southampton NHS Foundation Trust, Southampton, UK., Wenk MR; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.; Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, Singapore., Chan SY; Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, 1E Kent Ridge Road, NUHS Tower Block, Level 12, Singapore, 119228, Singapore. obgchan@nus.edu.sg.; Singapore Institute for Clinical Sciences, Agency for Science, Technology and Research, Singapore, Singapore. obgchan@nus.edu.sg.
Jazyk: angličtina
Zdroj: Molecular medicine (Cambridge, Mass.) [Mol Med] 2021 Aug 06; Vol. 27 (1), pp. 84. Date of Electronic Publication: 2021 Aug 06.
DOI: 10.1186/s10020-021-00344-w
Abstrakt: Background: Fetal docosahexaenoic acid (DHA) supply relies on preferential transplacental transfer, which is regulated by placental DHA lipid metabolism. Maternal hyperglycemia and obesity associate with higher birthweight and fetal DHA insufficiency but the role of placental DHA metabolism is unclear.
Methods: Explants from 17 term placenta were incubated with 13 C-labeled DHA for 48 h, at 5 or 10 mmol/L glucose treatment, and the production of 17 individual newly synthesized 13 C-DHA labeled lipids quantified by liquid chromatography mass spectrometry.
Results: Maternal BMI positively associated with 13 C-DHA-labeled diacylglycerols, triacylglycerols, lysophospholipids, phosphatidylcholine and phosphatidylethanolamine plasmalogens, while maternal fasting glycemia positively associated with five 13 C-DHA triacylglycerols. In turn, 13 C-DHA-labeled phospholipids and triacylglycerols positively associated with birthweight centile. In-vitro glucose treatment increased most 13 C-DHA-lipids, but decreased 13 C-DHA phosphatidylethanolamine plasmalogens. However, with increasing maternal BMI, the magnitude of the glucose treatment induced increase in 13 C-DHA phosphatidylcholine and 13 C-DHA lysophospholipids was curtailed, with further decline in 13 C-DHA phosphatidylethanolamine plasmalogens. Conversely, with increasing birthweight centile glucose treatment induced increases in 13 C-DHA triacylglycerols were exaggerated, while glucose treatment induced decreases in 13 C-DHA phosphatidylethanolamine plasmalogens were diminished.
Conclusions: Maternal BMI and glycemia increased the production of different placental DHA lipids implying impact on different metabolic pathways. Glucose-induced elevation in placental DHA metabolism is moderated with higher maternal BMI. In turn, findings of associations between many DHA lipids with birthweight suggest that BMI and glycemia promote fetal growth partly through changes in placental DHA metabolism.
(© 2021. The Author(s).)
Databáze: MEDLINE
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