| Autor: |
Hemm F; Department of Trauma, Hand and Reconstructive Surgery, University Hospital of Giessen, Rudolf-Buchheim-Str. 7, 35392 Giessen, Germany.; Experimental Trauma Surgery, Justus-Liebig-University Giessen, Aulweg 128, 35392 Giessen, Germany., Fijak M; Department of Anatomy and Cell Biology, Justus-Liebig-University Giessen, Aulweg 123, 35385 Giessen, Germany., Belikan J; Laboratory of Experimental Radiology, Justus-Liebig-University Giessen, Schubertstrasse 81, 35392 Giessen, Germany., Kampschulte M; Laboratory of Experimental Radiology, Justus-Liebig-University Giessen, Schubertstrasse 81, 35392 Giessen, Germany., El Khassawna T; Experimental Trauma Surgery, Justus-Liebig-University Giessen, Aulweg 128, 35392 Giessen, Germany., Pilatz A; Department of Urology, Pediatric Urology and Andrology, University Hospital of Giessen, Rudolf-Buchheim-Straße 7, 35392 Giessen, Germany., Heiss C; Department of Trauma, Hand and Reconstructive Surgery, University Hospital of Giessen, Rudolf-Buchheim-Str. 7, 35392 Giessen, Germany.; Experimental Trauma Surgery, Justus-Liebig-University Giessen, Aulweg 128, 35392 Giessen, Germany., Lips KS; Experimental Trauma Surgery, Justus-Liebig-University Giessen, Aulweg 128, 35392 Giessen, Germany. |
| Abstrakt: |
Investigations in male patients with fertility disorders revealed a greater risk of osteoporosis. The rodent model of experimental autoimmune-orchitis (EAO) was established to analyze the underlying mechanisms of male infertility and causes of reduced testosterone concentration. Hence, we investigated the impact of testicular dysfunction in EAO on bone status. Male mice were immunized with testicular homogenate in adjuvant to induce EAO ( n = 5). Age-matched mice were treated with adjuvant alone (adjuvant, n = 6) or remained untreated (control, n = 7). Fifty days after the first immunization specimens were harvested. Real-time reverse transcription-PCR indicated decreased bone metabolism by alkaline phosphatase and Cathepsin K as well as remodeling of cell-contacts by Connexin-43. Micro computed tomography demonstrated a loss of bone mass and mineralization. These findings were supported by histomorphometric results. Additionally, biomechanical properties of femora in a three-point bending test were significantly altered. In summary, the present study illustrates the induction of osteoporosis in the investigated mouse model. However, results suggest that the major effects on bone status were mainly caused by the complete Freund's adjuvant rather than the autoimmune-orchitis itself. Therefore, the benefit of the EAO model to transfer laboratory findings regarding bone metabolism in context with orchitis into a clinical application is limited. |
