New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis .

Autor: Karamysheva T; Institute of Cytology and Genetics, The Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia., Romanenko S; Institute of Molecular and Cellular Biology, The Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia., Makunin A; Wellcome Sanger Institute, Cambridge CB10 1SA, UK., Rajičić M; Institute for Biological Research 'Siniša Stanković', National Institute of Republic of Serbia, 11060 Belgrade, Serbia., Bogdanov A; Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 119991 Moscow, Russia., Trifonov V; Institute of Molecular and Cellular Biology, The Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.; Department of Genetic Technologies, Novosibirsk State University, 630090 Novosibirsk, Russia., Blagojević J; Institute for Biological Research 'Siniša Stanković', National Institute of Republic of Serbia, 11060 Belgrade, Serbia., Vujošević M; Institute for Biological Research 'Siniša Stanković', National Institute of Republic of Serbia, 11060 Belgrade, Serbia., Orishchenko K; Institute of Cytology and Genetics, The Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.; Department of Genetic Technologies, Novosibirsk State University, 630090 Novosibirsk, Russia., Rubtsov N; Institute of Cytology and Genetics, The Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.; Department of Genetic Technologies, Novosibirsk State University, 630090 Novosibirsk, Russia.
Jazyk: angličtina
Zdroj: Cells [Cells] 2021 Jul 19; Vol. 10 (7). Date of Electronic Publication: 2021 Jul 19.
DOI: 10.3390/cells10071819
Abstrakt: The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that heterochromatic compartments are not randomly localized in a nucleus. The purpose of this work was to study the organization and three-dimensional spatial arrangement of Bs in the interphase nucleus. Using microdissection of Bs and autosome centromeric heterochromatic regions of the yellow-necked mouse ( Apodemus flavicollis ) we obtained DNA probes for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained here B-specific DNA probes and autosomal C-positive pericentromeric region-specific probes further corroborated the previously stated hypothesis about the pseudoautosomal origin of the additional chromosomes of this species. Analysis of the spatial organization of the Bs demonstrated the peripheral location of B-specific chromatin within the interphase nucleus and feasible contact with the nuclear envelope (similarly to pericentromeric regions of autosomes and sex chromosomes). It is assumed that such interaction is essential for the regulation of nuclear architecture. It also points out that Bs may follow the same mechanism as sex chromosomes to avoid a meiotic checkpoint.
Databáze: MEDLINE
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