Pluripotent stem cell-derived corneal endothelial cells as an alternative to donor corneal endothelium in keratoplasty.

Autor:	Ali M; The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA., Khan SY; The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA., Gottsch JD; The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA., Hutchinson EK; Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Khan A; Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Riazuddin SA; The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA. Electronic address: riazuddin@jhmi.edu.
Jazyk:	angličtina
Zdroj:	Stem cell reports [Stem Cell Reports] 2021 Sep 14; Vol. 16 (9), pp. 2320-2335. Date of Electronic Publication: 2021 Aug 05.
DOI:	10.1016/j.stemcr.2021.07.008
Abstrakt:	Here, we evaluate the efficacy of cryopreserved human embryonic stem cell (hESC)-derived corneal endothelial cells (CECs) to form a functional monolayer of corneal endothelium (CE) in rabbits and monkeys. We injected cryopreserved hESC-derived CECs into the anterior chamber of rabbits and monkeys either immediately after mechanical scraping of the central CE or a few days later when corneal edema developed. All preclinical models developed deturgesced and clear corneas following the injection of cryopreserved hESC-derived CECs and remained comparable to the corneas of the untreated eye. Confocal scanning microscopy confirmed an intact structure of hexagonal/polygonal cells and immunohistochemical analysis illustrated a monolayer expressing barrier and pump function proteins in the regenerated CE. The necropsy examination confirmed no remarkable change in multiple tissues assessed for teratoma formation. In conclusion, our data demonstrate the efficacy of cryopreserved hESC-derived CECs to form a functional CE on the denuded Descemet's membrane. (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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