A nanoscale DNA force spectrometer capable of applying tension and compression on biomolecules.

Autor:	Wang Y; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA., Le JV; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.; Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA., Crocker K; Department of Physics, The Ohio State University, Columbus, OH 43210, USA., Darcy MA; Department of Physics, The Ohio State University, Columbus, OH 43210, USA., Halley PD; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA., Zhao D; Department of Physics, The Ohio State University, Columbus, OH 43210, USA., Andrioff N; Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA., Croy C; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA., Poirier MG; Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA.; Department of Physics, The Ohio State University, Columbus, OH 43210, USA.; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA., Bundschuh R; Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA.; Department of Physics, The Ohio State University, Columbus, OH 43210, USA.; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.; Division of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USA., Castro CE; Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.; Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, USA.
Jazyk:	angličtina
Zdroj:	Nucleic acids research [Nucleic Acids Res] 2021 Sep 07; Vol. 49 (15), pp. 8987-8999.
DOI:	10.1093/nar/gkab656
Abstrakt:	Single molecule force spectroscopy is a powerful approach to probe the structure, conformational changes, and kinetic properties of biological and synthetic macromolecules. However, common approaches to apply forces to biomolecules require expensive and cumbersome equipment and relatively large probes such as beads or cantilevers, which limits their use for many environments and makes integrating with other methods challenging. Furthermore, existing methods have key limitations such as an inability to apply compressive forces on single molecules. We report a nanoscale DNA force spectrometer (nDFS), which is based on a DNA origami hinge with tunable mechanical and dynamic properties. The angular free energy landscape of the nDFS can be engineered across a wide range through substitution of less than 5% of the strand components. We further incorporate a removable strut that enables reversible toggling of the nDFS between open and closed states to allow for actuated application of tensile and compressive forces. We demonstrate the ability to apply compressive forces by inducing a large bend in a 249bp DNA molecule, and tensile forces by inducing DNA unwrapping of a nucleosome sample. These results establish a versatile tool for force spectroscopy and robust methods for designing nanoscale mechanical devices with tunable force application. (© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu