| Autor: |
Souza BMN; Laboratory of Photobiology Applied to Health, Research and Development Institute, University of Vale do Paraíba, Urbanova 2911, Brazil., Pinto JG; Laboratory of Photobiology Applied to Health, Research and Development Institute, University of Vale do Paraíba, Urbanova 2911, Brazil., Pereira AHC; Laboratory of Photobiology Applied to Health, Research and Development Institute, University of Vale do Paraíba, Urbanova 2911, Brazil., Miñán AG; Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata 1900, Argentina., Ferreira-Strixino J; Laboratory of Photobiology Applied to Health, Research and Development Institute, University of Vale do Paraíba, Urbanova 2911, Brazil. |
| Jazyk: |
angličtina |
| Zdroj: |
Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2021 Jul 17; Vol. 10 (7). Date of Electronic Publication: 2021 Jul 17. |
| DOI: |
10.3390/antibiotics10070869 |
| Abstrakt: |
Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine ® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm 2 . The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference ( p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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